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PMID:16816199
| Citation |
Alonso-Casajús, N, Dauvillée, D, Viale, AM, Muñoz, FJ, Baroja-Fernández, E, Morán-Zorzano, MT, Eydallin, G, Ball, S and Pozueta-Romero, J (2006) Glycogen phosphorylase, the product of the glgP Gene, catalyzes glycogen breakdown by removing glucose units from the nonreducing ends in Escherichia coli. J. Bacteriol. 188:5266-72 |
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| Abstract |
To understand the biological function of bacterial glycogen phosphorylase (GlgP), we have produced and characterized Escherichia coli cells with null or altered glgP expression. glgP deletion mutants (DeltaglgP) totally lacked glycogen phosphorylase activity, indicating that all the enzymatic activity is dependent upon the glgP product. Moderate increases of glycogen phosphorylase activity were accompanied by marked reductions of the intracellular glycogen levels in cells cultured in the presence of glucose. In turn, both glycogen content and rates of glycogen accumulation in DeltaglgP cells were severalfold higher than those of wild-type cells. These defects correlated with the presence of longer external chains in the polysaccharide accumulated by DeltaglgP cells. The overall results thus show that GlgP catalyzes glycogen breakdown and affects glycogen structure by removing glucose units from the polysaccharide outer chains in E. coli. |
| Links |
PubMed PMC1539952 Online version:10.1128/JB.01566-05 |
| Keywords |
Base Sequence; DNA Primers; Escherichia coli/cytology; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Genotype; Glucose/metabolism; Glycogen/metabolism; Glycogen Phosphorylase/genetics; Glycogen Phosphorylase/metabolism; Kinetics; Molecular Sequence Data; Polysaccharides, Bacterial/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0008184: glycogen phosphorylase activity |
ECO:0000315: |
F |
The gene glgP encodes the protein GlgP. This protein is responsible for the breakdown of glycogen. In fig 1 part B, the ΔglgP phenotype shows that the levels of glycogen does not decline, ie glycogen isn’t being broken down. |
complete | ||||
See also
References
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