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User:Cherylrood

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CACAO Spring 2017

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorACIBA:C9D9L22017-02-17 15:22:15 CSTGO:0009405 pathogenesis (P)PMID:25466824ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 6

These two graphs showed that both Wild-type and overexpressed uspA (Universal Stress Protein A) contributed to Acinetobacter baumannii (ATCC 17978) pneumonia pathogenesis and lethality in sepsis.

challenge
updatedbyinstructorSALTY:USPA2017-02-26 20:54:27 CSTGO:0009405 pathogenesis (P)PMID:17081727ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 7A At a dosage of 10^6 Salmonella typhimurium C5, M29 (uspA mutant) was significantly less virulent compared to the wild type

challenge
updatedbyinstructorNOSS1:Q8YP052017-02-26 23:00:29 CSTGO:1905157 positive regulation of photosynthesis (P)PMID:27102494ECO:0000315 mutant phenotype evidence used in manual assertion

Table 1.

Alkylhydroperoxide reductase (ahpC) in Anabaena species (PCC7120) Table comparing PS I, PS II activity and respiration in wild type, AnΔahpC and An + ahpC.

challenge
updatedbyinstructorNOSS1:Q8YP052017-03-05 15:37:35 CSTGO:0016163 nitrogenase activity (F)PMID:27102494ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 4.

Nitrogenase activity comparing wild type, Alkylhydroperoxide reductase(ahpC) An + ahpC and AnΔahpC strains of Anabaena species (PCC7120) under control and methyl viologen stress.

challenge
acceptableEDWTA:A2TEZ42017-03-19 17:38:51 CDTGO:0040010 positive regulation of growth rate (P)PMID:27102497ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 1.

Growth analysis of Edwardsiella tarda TX01 showed faster growth rate than TX01Δfur (ferric uptake regulator) mutant

challenge
acceptableEDWTA:A2TEZ42017-03-19 17:41:13 CDTGO:1900705 negative regulation of siderophore biosynthetic process (P)PMID:27102497ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 2 Chromoazurol S (CAS) assay compares siderophore production of Edwardsiella tarda TX01 and TX01Δfur. TX01Δfur (Ferric uptake regulator) mutant had increased siderophore production.

challenge
acceptableEDWTA:A2TEZ42017-03-24 01:07:42 CDTGO:0009405 pathogenesis (P)PMID:27102497ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 4a. In vivo infection study: Turbot (flatfish) were infected with a dose of Edwardsiella tarda TX01, TX01Δfur, or TX01ΔfurC. Plate counts of bacteria recovered from organs of TX01Δfur-infected fish were significantly lower than the TX01-infected fish at 24 and 48 hours post infection.

challenge
acceptableECOLX:F5AP032017-03-25 23:37:04 CDTGO:0009405 pathogenesis (P)PMID:22565007ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 3. Recovered bacteria in the brains of infected ducks was significantly reduced for the ∆ibeB mutant strain Escherichia coli DE205B4 compared to wild-type strain DE205B

challenge
acceptableECOLX:Q6X9722017-03-25 23:42:40 CDTGO:0009405 pathogenesis (P)PMID:20974831ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 4. The Escherichia coli (DE205M) ∆ibeA mutant-infected ducks had significantly lower bacteria loads isolated from lungs and brains of ducks compared to those infected with wild type E. coli (DE205B).

challenge
acceptableECOLX:A0A152UJI22017-03-25 23:47:29 CDTGO:0009405 pathogenesis (P)PMID:25461694ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 4 Colonization of the Escherichia coli (E058 strain) ∆rstA mutant was significant lower than those of the wild-type E058 strain in the internal organs of chickens.

challenge
acceptableECOLX:A0A085PA382017-03-25 23:50:21 CDTGO:0009405 pathogenesis (P)PMID:23504015ECO:0000315 mutant phenotype evidence used in manual assertion

Table 3. 100% Death in WT avian Escherichia coli (serotype O2 APEC E058) compared to 0% death of the E5058 ∆rfaH mutant after air sac infection

challenge
acceptableECOLX:E2QL902017-03-25 23:54:29 CDTGO:0009405 pathogenesis (P)PMID:25768126ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 3 Survival assay showed that

seven-day-old ducks inoculated with Escherichia coli (DE205B) had lower survival rates than ducks inoculated with E. coli (DE205B) ΔibeR mutant

challenge

acceptable:8
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Cherylrood

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorCherylrood,
Team Voldemort
ACIBA:C9D9L2GO:0009405 - pathogenesis (P)PMID:25466824ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2

fixed by Cherylrood
unacceptableCRoss2008,
Team Dank Genes
MOUSE:GBRDGO:0051932 - synaptic transmission, GABAergic (P)PMID:25339733ECO:0000314 direct assay evidence used in manual assertionchallengeC: 4
updatedbyinstructorCRoss2008,
Team Dank Genes
MOUSE:GBRDGO:0030424 - axon (C)PMID:25339733ECO:0000314 direct assay evidence used in manual assertionchallengeC: 5

fixed by Cherylrood

2 annotations fixed by Cherylrood

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