PMID:27102497

Citation	Hu, YH and Sun, L (2016) The global regulatory effect of Edwardsiella tarda Fur on iron acquisition, stress resistance, and host infection: A proteomics-based interpretation. J Proteomics 140:100-10
Abstract	Ferric uptake regulator (Fur) is an important transcriptional regulator of Gram-negative bacteria. Edwardsiella tarda is a severe fish bacterial pathogen with a broad host range that includes humans. In this study, we examined the regulatory function of Fur in E. tarda via a proteomic approach. Compared to the wild type TX01, the fur mutant TX01Δfur exhibited (i) retarded growth, (ii) enhanced siderophore production, (iii) increased acid tolerance, which is in contrast to observations in other bacterial species, (iv) decreased survival against oxidative stress and host serum, (v) impaired ability to inhibit host immune response, (vi) attenuated tissue infectivity and overall virulence. The deficiency of TX01Δfur was rescued by introduction of an exogenous fur gene. iTRAQ-based comparative proteomic analysis of TX01Δfur and TX01 identified 89 differentially expressed proteins that cover a wide range of functional categories including those affected by fur mutation. In addition, 16 proteins were identified for the first time to be regulated by Fur in Gram-negative bacteria. These results provide the first protein-based interpretation of the global impact of Fur on the physiology and infectivity of E. tarda.
Links	PubMed Online version:10.1016/j.jprot.2016.04.005
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
EDWTA:A2TEZ4		GO:0040010: positive regulation of growth rate	ECO:0000315:		P		Fig. 1. Growth analysis of Edwardsiella tarda TX01 showed faster growth rate than TX01Δfur (ferric uptake regulator) mutant	complete CACAO 12323
EDWTA:A2TEZ4		GO:1900705: negative regulation of siderophore biosynthetic process	ECO:0000315:		P		Fig. 2 Chromoazurol S (CAS) assay compares siderophore production of Edwardsiella tarda TX01 and TX01Δfur. TX01Δfur (Ferric uptake regulator) mutant had increased siderophore production.	complete CACAO 12324
EDWTA:A2TEZ4		GO:0009405: pathogenesis	ECO:0000315:		P		Fig. 4a. In vivo infection study: Turbot (flatfish) were infected with a dose of Edwardsiella tarda TX01, TX01Δfur, or TX01ΔfurC. Plate counts of bacteria recovered from organs of TX01Δfur-infected fish were significantly lower than the TX01-infected fish at 24 and 48 hours post infection.	complete CACAO 12348

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