PMID:22565007

Citation	Wang, S, Shi, Z, Xia, Y, Li, H, Kou, Y, Bao, Y, Dai, J and Lu, C (2012) IbeB is involved in the invasion and pathogenicity of avian pathogenic Escherichia coli. Vet. Microbiol. 159:411-9
Abstract	The ibeB gene in neonatal meningitis Escherichia coli (NMEC) contribute to the penetration of human brain microvascular endothelial cells (HBMECs). However, whether IbeB plays a role in avian pathogenic E. coli (APEC) infection remains unclear. Thus, this study was conducted to investigate the distribution of the ibeB gene in Chinese APEC strains and examine whether IbeB is involved in APEC pathogenicity. The ibeB gene was found in all 100 detected E. coli isolates with over 97% sequence homology. These results indicated that ibeB is a conserved E. coli gene irrelevant of pathotypes. To determine the role of ibeB in APEC pathogenicity, an ibeB mutant of strain DE205B was constructed and characterized. The inactivation of ibeB resulted in reduced invasion capacity towards DF-1 cells and defective virulence in animal models as compared to the wild-type strain. Animal infection experiments revealed that loss of ibeB decreased APEC colonization and invasion capacity in brains and lungs. These virulence-related phenotypes were partially recoverable by genetic complementation. Reduced expression levels of invasion- and adhesion-associated genes in ibeB mutant could be major reasons as evidenced by reduced ibeA and ompA expression. These results indicate that IbeB is involved in APEC invasion and pathogenicity.
Links	PubMed Online version:10.1016/j.vetmic.2012.04.015
Keywords	Animals; Bacterial Adhesion; Chick Embryo; Ducks; Escherichia coli/classification; Escherichia coli/genetics; Escherichia coli/isolation & purification; Escherichia coli/pathogenicity; Escherichia coli Infections/pathology; Escherichia coli Infections/veterinary; Escherichia coli Proteins/genetics; Fibroblasts/microbiology; Humans; Membrane Proteins/genetics; Poultry Diseases/microbiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ECOLX:F5AP03		GO:0009405: pathogenesis	ECO:0000315:		P		Fig 3. Recovered bacteria in the brains of infected ducks was significantly reduced for the ∆ibeB mutant strain Escherichia coli DE205B4 compared to wild-type strain DE205B	complete CACAO 12374

References

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