PMID:27102494

Citation	Shrivastava, AK, Pandey, S, Yadav, S, Mishra, Y, Singh, PK, Rai, R, Singh, S, Rai, S and Rai, LC (2016) Comparative proteomics of wild type, An+ahpC and An∆ahpC strains of Anabaena sp. PCC7120 demonstrates AhpC mediated augmentation of photosynthesis, N2-fixation and modulation of regulatory network of antioxidative proteins. J Proteomics 140:81-99
Abstract	Alkylhydroperoxide reductase (AhpC), a 1-Cys peroxiredoxin is well known for maintaining the cellular homeostasis. Present study employs proteome approach to analyze and compare alterations in proteome of Anabaena PCC7120 in overexpressing (An+ahpC), deletion (An∆ahpC) and its wild type. 2-DE based analysis revealed that the major portion of identified protein belongs to energy metabolism, protein folding, modification and stress related proteins and carbohydrate metabolism. The two major traits discernible from An+ahpC were (i) augmentation of photosynthesis and nitrogen fixation (ii) modulation of regulatory network of antioxidative proteins. Increased accumulation of proteins of light reaction, dark reaction, pentose phosphate pathway and electron transfer agent FDX for nitrogenase in An+ahpC and their simultaneous downregulation in AnΔahpC demonstrates its role in augmenting photosynthesis and nitrogen fixation. Proteomic data was nicely corroborated with physiological, biochemical parameters displaying upregulation of nitrogenase (1.6 fold) PSI (1.08) and PSII (2.137) in An+ahpC. Furthermore, in silico analysis not only attested association of AhpC with peroxiredoxins but also with other players of antioxidative defense system viz. thioredoxin and thioredoxin reductase. Above mentioned findings are in agreement with 33-40% and 40-60% better growth performance of An+ahpC over wild type and An∆ahpC respectively under abiotic stresses, suggesting its role in maintenance of metabolic machinery under stress.
Links	PubMed Online version:10.1016/j.jprot.2016.04.004
Keywords
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
NOSS1:Q8YP05		GO:1905157: positive regulation of photosynthesis	ECO:0000315:		P		Table 1. Alkylhydroperoxide reductase (ahpC) in Anabaena species (PCC7120) Table comparing PS I, PS II activity and respiration in wild type, AnΔahpC and An + ahpC.	complete CACAO 12296
NOSS1:Q8YP05		GO:0016163: nitrogenase activity	ECO:0000315:		F		Fig. 4. Nitrogenase activity comparing wild type, Alkylhydroperoxide reductase(ahpC) An + ahpC and AnΔahpC strains of Anabaena species (PCC7120) under control and methyl viologen stress.	complete CACAO 12312

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