User:Cakate1

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	BPT5:DIT	2017-04-07 13:15:47 CDT	GO:0098025 virus tail, baseplate (C)	PMID:24155371	ECO:0000314 direct assay evidence used in manual assertion	Immunoelectron microscopy images displayed in Figure 1 show the pb9 protein localized in the upper part of the tail of the T5 phage. Through the use of IgG crosslinking, pb9 is proposed to be part of the adsorption device in the baseplate of the virus tail.	challenge
acceptable	9CAUD:Q9G097	2017-04-09 20:01:24 CDT	GO:0098026 virus tail, tube (C)	PMID:27824135	ECO:0000315 mutant phenotype evidence used in manual assertion	A series of different mutations were made in the TMP of this phage, including in the N-termini, C-termini, and hydrophobic regions of the protein. Figure 2 shows that mutations in the N and C-termini resulted in abnormal phage assembly, and hydrophobic region mutations resulted in tail length reduction. Therefore, the TMP is determined to be an important part of the tube in the viral tail.	challenge
acceptable	9CAUD:R4JMG7	2017-04-09 20:21:18 CDT	GO:0008233 peptidase activity (F)	PMID:27637947	ECO:0000314 direct assay evidence used in manual assertion	The M15A peptidase is shown to have peptidase lytic activity against E.coli host cells. Figure 3 shows peptidase activity by plotting it against the optical density of the host cells. The data shows a large reduction in the turbidity of the host cells through the activity of the M15A protein.	challenge
acceptable	9CAUD:H2D0G4	2017-04-09 20:45:04 CDT	GO:0016787 hydrolase activity (F)	PMID:22289622	ECO:0000314 direct assay evidence used in manual assertion	In one of the experiments described, they performed an endolysin assay to test the antimicrobial spectrum of this protein. Table 2 displays the relative lysis activity of SPN1S against certain Gram-positive and Gram-negative bacterial strains. The table shows SPN1S's high relative lysis activity against all the E.coli strains. These results provide evidence that the SPN1S protein has a hydrolase function.	challenge
acceptable	BPT5:DECO	2017-04-12 09:52:53 CDT	GO:0098021 viral capsid, decoration (C)	PMID:28165000	ECO:0000314 direct assay evidence used in manual assertion	An association assay was performed to test the decoration capabilities of pb10 on the T5 capsid. Figure 4 shows that the N-terminal domain of pb10 is responsible for interacting with the capsid. Figure 5 further exhibits a high affinity between the protein and the capsid through an SPR experiment. Overall, these data provide evidence for the decorating capabilities of pb10.	challenge
updatedbyinstructor	BPT5:HEL10	2017-04-17 10:47:21 CDT	GO:0032508 DNA duplex unwinding (P)	PMID:28009009	ECO:0000314 direct assay evidence used in manual assertion	Figure 3 shows how d10 protein functions to unwind DNA. It uses partially homologous Holliday junction substrates and results in two products, including fork DNA and recombined duplex DNA. These gels prove that d10 can act as a DNA helicase.	challenge
acceptable	BPP2:HOLIN	2017-04-26 10:09:38 CDT	GO:0034290 holin activity (F)	PMID:23335412	ECO:0000314 direct assay evidence used in manual assertion	Figure 2 displays the results of expressing the holin gene in the lambda phage and testing its lysing abilities on E.coli. The activity assay shows that the holin reduces turbidity in the E.coli and thereby has the lytic properties of a holin.	challenge
acceptable	BPP2:ANTIH	2017-04-26 10:16:10 CDT	GO:1901218 negative regulation of holin activity (P)	PMID:23335412	ECO:0000314 direct assay evidence used in manual assertion	Figure 4B shows the results of expressing the LysA gene on the activity of the Y holin. The incorporation of LysA resulted in a delay of the lysis activity of holin Y, thus indicating its negative regulation.	challenge

acceptable:7
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Cakate1

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