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9CAUD:Q9G097

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Species (Taxon ID) Lactococcus phage TP901-1. (35345)
Gene Name(s) tmp (ECO:0000313 with EMBL:AAK38062.1)
Protein Name(s) TMP (ECO:0000313 with EMBL:AAK38062.1)

Tape measure protein (ECO:0000313 with EMBL:AAG32164.1)

External Links
UniProt Q9G097
EMBL AF252967
AF304433
RefSeq NP_112708.1
GeneID 921037
KEGG vg:921037
GO GO:0016021
InterPro IPR010090
Pfam PF10145
TIGRFAMs TIGR01760

Annotations

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0016020

membrane

GO_REF:0000038

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0472

C

Seeded From UniProt

GO:0016021

integral component of membrane

GO_REF:0000038

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0812

C

Seeded From UniProt

Contributes to

GO:0098004

virus tail fiber assembly

PMID:27824135[1]

IMP: Inferred from Mutant Phenotype

P

56 mutants were created to characterize which domains of TP901-1's tape measure gene (TMP) were essential for infection of the phage and it's tail assembly. Through this experiment, TP901-1's N-terminal 154 amino acid residues, C-terminal 60 residues, and hydrophobic region were revealed to be required for TP901's infectivity. This is shown in fig.2 in the paper, where removing 30 amino acids from both N and C-termini cause phage assembly to be aberrant, while removal of the hydrophobic region causes tai length reduction.

complete
CACAO 12553

GO:0098026

virus tail, tube

PMID:27824135[1]

IMP: Inferred from Mutant Phenotype

C

A series of different mutations were made in the TMP of this phage, including in the N-termini, C-termini, and hydrophobic regions of the protein. Figure 2 shows that mutations in the N and C-termini resulted in abnormal phage assembly, and hydrophobic region mutations resulted in tail length reduction. Therefore, the TMP is determined to be an important part of the tube in the viral tail.

complete
CACAO 12588

Contributes to

GO:0098003

viral tail assembly

PMID:27824135[1]

IMP: Inferred from Mutant Phenotype

P

The 29 repeats of the tape measure protein are essential to virion structure. Deletion mutants for repeat copies were constructed, then had tail length measured through electron microscopy. Authors found that, with increased deletion of repeat sequences, the phage tail shortened (Table 2). For example, normal phage tail length is 118nm, but repeat 1-29 deletion mutants had a tail of 65 nm.

complete
CACAO 13108

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 1.2 Mahony, J et al. (2016) Functional and structural dissection of the tape measure protein of lactococcal phage TP901-1. Sci Rep 6 36667 PubMed GONUTS page