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PMID:27824135

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Citation

Mahony, J, Alqarni, M, Stockdale, S, Spinelli, S, Feyereisen, M, Cambillau, C and Sinderen, DV (2016) Functional and structural dissection of the tape measure protein of lactococcal phage TP901-1. Sci Rep 6:36667

Abstract

The tail tape measure protein (TMP) of tailed bacteriophages (also called phages) dictates the tail length and facilitates DNA transit to the cell cytoplasm during infection. Here, a thorough mutational analysis of the TMP from lactococcal phage TP901-1 (TMPTP901-1) was undertaken. We generated 56 mutants aimed at defining TMPTP901-1 domains that are essential for tail assembly and successful infection. Through analysis of the derived mutants, we determined that TP901-1 infectivity requires the N-terminal 154 aa residues, the C-terminal 60 residues and the first predicted hydrophobic region of TMPTP901-1 as a minimum. Furthermore, the role of TMPTP901-1 in tail length determination was visualized by electron microscopic imaging of TMP-deletion mutants. The inverse linear correlation between the extent of TMPTP901-1-encoding gene deletions and tail length of the corresponding virion provides an estimate of TMPTP901-1 regions interacting with the connector or involved in initiator complex formation. This study represents the most thorough characterisation of a TMP from a Gram-positive host-infecting phage and provides essential advances to understanding its role in virion assembly, morphology and infection.

Links

PubMed PMC5099701 Online version:10.1038/srep36667

Keywords


Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

9CAUD:Q9G097

Contributes to

GO:0098004

virus tail fiber assembly

IMP: Inferred from Mutant Phenotype

P

56 mutants were created to characterize which domains of TP901-1's tape measure gene (TMP) were essential for infection of the phage and it's tail assembly. Through this experiment, TP901-1's N-terminal 154 amino acid residues, C-terminal 60 residues, and hydrophobic region were revealed to be required for TP901's infectivity. This is shown in fig.2 in the paper, where removing 30 amino acids from both N and C-termini cause phage assembly to be aberrant, while removal of the hydrophobic region causes tai length reduction.

complete
CACAO 12553

9CAUD:Q9G097

GO:0098026

virus tail, tube

IMP: Inferred from Mutant Phenotype

C

A series of different mutations were made in the TMP of this phage, including in the N-termini, C-termini, and hydrophobic regions of the protein. Figure 2 shows that mutations in the N and C-termini resulted in abnormal phage assembly, and hydrophobic region mutations resulted in tail length reduction. Therefore, the TMP is determined to be an important part of the tube in the viral tail.

complete
CACAO 12588

9CAUD:Q9G097

Contributes to

GO:0098003

viral tail assembly

IMP: Inferred from Mutant Phenotype

P

The 29 repeats of the tape measure protein are essential to virion structure. Deletion mutants for repeat copies were constructed, then had tail length measured through electron microscopy. Authors found that, with increased deletion of repeat sequences, the phage tail shortened (Table 2). For example, normal phage tail length is 118nm, but repeat 1-29 deletion mutants had a tail of 65 nm.

complete
CACAO 13108

Notes

See also

References

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