GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:28165000
| Citation |
Vernhes, E, Renouard, M, Gilquin, B, Cuniasse, P, Durand, D, England, P, Hoos, S, Huet, A, Conway, JF, Glukhov, A, Ksenzenko, V, Jacquet, E, Nhiri, N, Zinn-Justin, S and Boulanger, P (2017) High affinity anchoring of the decoration protein pb10 onto the bacteriophage T5 capsid. Sci Rep 7:41662 |
|---|---|
| Abstract |
Bacteriophage capsids constitute icosahedral shells of exceptional stability that protect the viral genome. Many capsids display on their surface decoration proteins whose structure and function remain largely unknown. The decoration protein pb10 of phage T5 binds at the centre of the 120 hexamers formed by the major capsid protein. Here we determined the 3D structure of pb10 and investigated its capsid-binding properties using NMR, SAXS, cryoEM and SPR. Pb10 consists of an α-helical capsid-binding domain and an Ig-like domain exposed to the solvent. It binds to the T5 capsid with a remarkably high affinity and its binding kinetics is characterized by a very slow dissociation rate. We propose that the conformational exchange events observed in the capsid-binding domain enable rearrangements upon binding that contribute to the quasi-irreversibility of the pb10-capsid interaction. Moreover we show that pb10 binding is a highly cooperative process, which favours immediate rebinding of newly dissociated pb10 to the 120 hexamers of the capsid protein. In extreme conditions, pb10 protects the phage from releasing its genome. We conclude that pb10 may function to reinforce the capsid thus favouring phage survival in harsh environments. |
| Links |
PubMed PMC5292684 Online version:10.1038/srep41662 |
| Keywords |
|
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0098021: viral capsid, decoration |
ECO:0000314: |
C |
An association assay was performed to test the decoration capabilities of pb10 on the T5 capsid. Figure 4 shows that the N-terminal domain of pb10 is responsible for interacting with the capsid. Figure 5 further exhibits a high affinity between the protein and the capsid through an SPR experiment. Overall, these data provide evidence for the decorating capabilities of pb10. |
complete | ||||
Notes
See also
References
See Help:References for how to manage references in GONUTS.
