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User:Bmanieri

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CACAO Spring 2014

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableHUMAN:COGA12014-01-29 20:20:15 CSTGO:0008544 epidermis development (P)PMID:16754661ECO:0000314 direct assay evidence used in manual assertion

Epidermis

challenge
unacceptableYEAST:TAF142014-01-30 09:47:34 CSTGO:0005667 transcription factor complex (C)PMID:7995524ECO:0000314 direct assay evidence used in manual assertion

Figure 4E shows the precipitates that were electrophoresed in a SDS-10% poly- acrylamide gel. It shows by the overlap that yeast TAFs can be immunoprecipitated by antibodies against

Tfg3. Antibody against Tfg3 (lane 1) or mAb 12CA5 (lane 2) was used to immunoprecipitate yeast TAFs from a complex fraction.

challenge
unacceptableYEAST:TAF142014-01-30 10:05:52 CSTGO:0005674 transcription factor TFIIF complex (C)PMID:7995524ECO:0000314 direct assay evidence used in manual assertion

Figure 5 Depicts region 4.2 showing the sequence homology between Tfg2 of bacterial cr factors. Alignment of the carboxy-terminal residues of Tfg2 with the carboxy-terminal residues of E. coli and Bacillus subtilis.

challenge
unacceptableMOUSE:EGFLA2014-01-30 10:17:03 CSTGO:0031012 extracellular matrix (C)PMID:16141072ECO:0000314 direct assay evidence used in manual assertion

Figure 2B Shows strong evidence via tag mapping of 3′ end initiation was correlated with a short intergenic distance when in tail-to-tail orientation with a neighboring gene suggesting a possible role in an intergenic regulatory interaction.

challenge
unacceptableHUMAN:CFAD2014-01-30 10:33:39 CSTGO:0003817 complement factor D activity (F)PMID:00746ECO:0000314 direct assay evidence used in manual assertion

Figure 5 depects that overall, the tertiary structure of the S94Y/T214S/S215W mutant is very similar to that of native factor D.

challenge
updatedbyinstructorHUMAN:COGA12014-01-30 10:51:37 CSTGO:0033622 integrin activation (P)PMID:16754661ECO:0000314 direct assay evidence used in manual assertion

Figure 3 depicts via fluorescence tagging that collagen XVI induces recruitment of integrins α1β1 and α2β1 into focal adhesion plaques.

challenge
updatedbyinstructorHUMAN:COGA12014-01-30 10:59:38 CSTGO:0033627 cell adhesion mediated by integrin (P)PMID:16754661ECO:0000314 direct assay evidence used in manual assertion

Figure 2 depicts (via cell adhesion absorbance) cell attachment to collagen XVI and its inhibition by monoclonal antibodies against α1 and α2 integrin subunits.

challenge

acceptable:0
unacceptable:5
requires_changes:0
flagged:0

Annotations challenged by Bmanieri

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
unacceptableSirons,
Team Takin This Outside
HUMAN:TAUGO:0007015 - actin filament organization (P)GO_REF:0000019ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2
unacceptableBmanieri,
Team Chicken Day
HUMAN:CFADGO:0003817 - obsolete complement factor D activity (F)PMID:00746ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2

0 annotations fixed by Bmanieri

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