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PMID:16754661

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Citation

Eble, JA, Kassner, A, Niland, S, Mörgelin, M, Grifka, J and Grässel, S (2006) Collagen XVI harbors an integrin alpha1 beta1 recognition site in its C-terminal domains. J. Biol. Chem. 281:25745-56

Abstract

Collagen XVI is integrated tissue-dependently into distinct fibrillar aggregates, such as D-banded cartilage fibrils and fibrillin-1-containing microfibrils. In skin, the distribution of collagen XVI overlaps that of the collagen-binding integrins alpha1 beta1 and alpha2 beta1. Basal layer keratinocytes express integrin alpha2 beta1, whereas integrin alpha1 beta1 occurs in smooth muscle cells surrounding blood vessels, in hair follicles, and on adipocytes. Cells bearing the integrins alpha1 beta1 and alpha2 beta1 attach and spread on recombinant collagen XVI. Furthermore, collagen XVI induces the recruitment of these integrins into focal adhesion plaques, a principal step in integrin signaling. Of potential physiological relevance, these integrin-collagen XVI interactions may connect cells with specialized fibrils, thus contributing to the organization of fibrillar and cellular components within connective tissues. In cell-free binding assays, collagen XVI is more avidly bound by alpha1 beta1 integrin than by alpha2 beta1 integrin. Both integrins interact with collagen XVI via the A domain of their alpha subunits. A tryptic collagen XVI fragment comprising the collagenous domains 1-3 is recognized by alpha1 beta1 integrin. Electron microscopy of complexes of alpha1 beta1 integrin with this tryptic collagen XVI fragment or with full-length collagen XVI revealed a unique alpha1 beta1 integrin-binding site within collagen XVI located close to its C-terminal end.

Links

PubMed Online version:10.1074/jbc.M509942200

Keywords

Adipocytes/metabolism; Animals; Binding Sites; Cell Adhesion; Collagen/chemistry; Integrin alpha1beta1/chemistry; Mice; Muscle, Smooth/cytology; Protein Binding; Protein Structure, Tertiary; Skin/pathology; Trypsin/chemistry

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:ITB1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q07092

F

Seeded From UniProt

complete

HUMAN:ITA2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q07092

F

Seeded From UniProt

complete

HUMAN:ITA1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q07092

F

Seeded From UniProt

complete

HUMAN:COGA1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P05556

F

Seeded From UniProt

complete

HUMAN:COGA1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P17301

F

Seeded From UniProt

complete

HUMAN:COGA1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P56199

F

Seeded From UniProt

complete

HUMAN:COGA1

GO:0008544: epidermis development

ECO:0000314:

P

Epidermis

complete
CACAO 9305

HUMAN:COGA1

GO:0033622: integrin activation

ECO:0000314:

P

Figure 3 depicts via fluorescence tagging that collagen XVI induces recruitment of integrins α1β1 and α2β1 into focal adhesion plaques.

complete
CACAO 9320

HUMAN:COGA1

GO:0033627: cell adhesion mediated by integrin

ECO:0000314:

P

Figure 2 depicts (via cell adhesion absorbance) cell attachment to collagen XVI and its inhibition by monoclonal antibodies against α1 and α2 integrin subunits.

complete
CACAO 9321

HUMAN:COGA1

involved_in

GO:0007229: integrin-mediated signaling pathway

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:COGA1

enables

GO:0005178: integrin binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:COGA1

involved_in

GO:0007155: cell adhesion

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:COGA1

involved_in

GO:0033627: cell adhesion mediated by integrin

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:COGA1

involved_in

GO:0033622: integrin activation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

See also

References

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