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Category:Team Blue A 2019

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableTHET2:SODMCColson, Team Blue A 20192019-04-13 13:49:44 CDTGO:0071793 bacillithiol biosynthetic process (P)PMID:30940703ECO:0001272 high-performance liquid chromatography evidence used in manual assertion

In Δsod Thermus thermophilus mutants, HPLC is used to observe a statistically significant drop in reduced bacillthiol pools (BSH), despite the total BSH (oxidized and reduced) concentrations remaining the same. Sod (superoxide dismutase) is a well-characterized antioxidant enzyme and its deletion, combined with the difference in reduced BSH levels, suggests Sod plays a role in BSH oxidation.

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acceptableBORBU:O51190ABatista, Team Blue A 20192019-03-31 18:19:32 CDTGO:0015972 guanosine pentaphosphate metabolic process (P)PMID:30478087ECO:0007110 thin layer chromatography evidence used in manual assertion

The authors generated a mutant strain of Borrelia burgdorferi with the dksA gene knocked out. Using thin-layer chromatography, they measured the levels of (p)ppGpp produced by both wild-type and ΔdksA mutants under starvation conditions (figure 7A). When these results were quantified and normalized to levels of (p)ppGpp+GTP, the authors found that the ΔdksA mutant produced significantly higher levels of (p)ppGpp than wild-type (figure 7B). Reinsertion of the dksA gene through a plasmid vector containing the dksA ORF (pDksA) restored production levels of (p)ppGpp comparable to that of the wild-type strain. This indicates that the dksA gene plays a role in regulation of guanosine pentaphosphate metabolism.

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acceptableMOUSE:DEN5BKAtanasoff, Team Blue A 20192019-03-09 15:10:17 CSTGO:1905885 positive regulation of triglyceride transport (P)PMID:30837651ECO:0005601 immunofluorescence confocal microscopy evidence used in manual assertion

In Figure 2G, there is a significant absence of apoB in dennd5b -/- mice, a protein required for enterocyte absorption of triglycerides (lipids), compared to dennd5b+/+ mice.

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Pages in category "Team Blue A 2019"

The following 3 pages are in this category, out of 3 total.


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