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GO:0015973guanosine pentaphosphate biosynthetic processPMID:30478087ECO:0007110 IMP: Inferred from Mutant PhenotypeBiological Process
The authors used a mutant DksA gene to portray the relationship between DksA and the up-regulation of (p)ppGpp levels in starvation-like conditions (Figure 7).
complete
This annotation made on page: BORBU:O51190
By: ABatista (group Team Blue A 2019) on 2019-03-31 18:19:32 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeCXie,
Team Red B 2019
2019-04-02 22:36:11 CDT

The note needs to differentiate between the mutant dksA and mutant dKsA pDksA strains. Mutant dksA is significantly different from WT, while dksA pDksA is not. Your current note only mentions mutant dksA compared to WT. It is also important to explain that Fig 7B is normalized to (p)ppGpp + GTP and does not directly represent (p)ppGpp levels, which could be inferred from your current note.

1
ChallengeMGuldan,
Team Green A 2019
2019-04-01 08:39:14 CDT

I think the note needs more detail and information about the experiment. How does the mutant DksA gene show that relationship? What test was actually done in figure 7?

2
Public
Assessment
Ivanerill2019-04-18 10:41:13 CDTNo notes given.Acceptable
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DanielRenfro2019-04-17 10:28:17 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0015972 guanosine pentaphosphate metabolic process PMID:30478087 ECO:0007110 IMP: Inferred from Mutant Phenotype P The authors generated a mutant strain of Borrelia burgdorferi with the dksA gene knocked out. Using thin-layer chromatography, they measured the levels of (p)ppGpp produced by both wild-type and ΔdksA mutants under starvation conditions (figure 7A). When these results were quantified and normalized to levels of (p)ppGpp+GTP, the authors found that the ΔdksA mutant produced significantly higher levels of (p)ppGpp than wild-type (figure 7B). Reinsertion of the dksA gene through a plasmid vector containing the dksA ORF (pDksA) restored production levels of (p)ppGpp comparable to that of the wild-type strain. This indicates that the dksA gene plays a role in regulation of guanosine pentaphosphate metabolism. complete
CACAO 13662
on BORBU:O51190
Flagged
Public
Assessment
Ivanerill2019-04-08 16:50:56 CDT

You chose a difficult figure for your annotation. The authors do not "demonstrate" that dksA is involved in "guanosine pentaphosphate biosynthetic process", just that it "might" be involved in its regulation. A bit tenuous overall.

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Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2019-04-03 09:35:00 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0015973 guanosine pentaphosphate biosynthetic process PMID:30478087 ECO:0007110 IMP: Inferred from Mutant Phenotype P The authors generated a mutant strain of Borrelia burgdorferi with the dskA gene knocked out. Using thin-layer chromatography, they measured the levels of (p)ppGpp produced by both wild-type and ΔdksA mutants under starvation conditions (figure 7A). When these results were quantified and normalized to levels of (p)ppGpp+GTP, the authors found that the ΔdksA mutant produced significantly higher levels of (p)ppGpp than wild-type (figure 7B). This indicates that the dskA gene may play a role in regulation of the biosynthesis of guanosine pentaphosphate. complete
CACAO 13662
on BORBU:O51190
Flagged