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Category:Team Red B 2019
|Status||Page||User||Date/Time||GO Term (Aspect)||Reference||Evidence||Notes||Links|
|CELJU:B3PDN7||RSpruill, Team Red B 2019||2019-04-10 10:55:50 CDT||GO:2000891 cellobiose metabolic process (P)||PMID:28118504||ECO:0001147 anion-exchange chromatography evidence used in manual assertion|
In Figure 2, wild type C. japonicus and cel3b, cel3Acel3B, and 4BG mutants were grown on cellobiose and glucose (control). The four strains showed no significant changes in glucose consumption during growth (Fig. 2A & 2B). However, the cel3b mutant showed a decreased rate of cellobiose consumption (Fig. 2C & 2D). This shows that cel3b has a role in efficient cellobiose utilization in C. japonicus.
|RHIME:Q92RW9||CXie, Team Red B 2019||2019-03-31 16:42:15 CDT||GO:0004673 protein histidine kinase activity (F)||PMID:17237174||ECO:0005641 IMP: Inferred from Mutant Phenotype|
B-glucuronidase activity was used to determine relative gene expression levels of uidA+ transcription fusion proteins, Smc00887 and Smc00888 (Fig 2). Smc00888 encodes a histidine kinase. During stationary phase, SMc00888 locus expression decreased three-fold in the cbrA::Tn5 mutant compared to WT.
|MOUSE:TS1R2||LWachhaus, Team Red B 2019||2019-03-24 21:58:33 CDT||GO:0033041 sweet taste receptor activity (F)||PMID:11509186||ECO:0001249 fluorescence evidence used in manual assertion|
To demonstrate specificity of the sweet taste receptor, the pathway of G-protein coupled receptors linked to the receptor was modified to increase intracellular calcium which was observed using the fluorescent FURA-2 calcium indicator dye. An increase in cellular calcium was observed (normalized to responses observed from the highest concentration per compound) in the sweet compounds GA-2, saccharin, acesulfame-K, and sucrose in the receptor combination T1R2+3 (Fig 6b), matching the biological thresholds observed in vivo (Fig 6a). Dose responses were not observed for bitter or umami compounds (Fig 6b). As intracellular calcium was not shown to increase upon exposure to any compound other than sugar and artificial sweeteners, the function of T1Rs as a sweet taste receptors is maintained.
|MOUSE:TS1R2||LWachhaus, Team Red B 2019||2019-03-10 15:06:43 CDT||GO:0033041 sweet taste receptor activity (F)||PMID:11509186||ECO:0001565 cell-based assay evidence|
Dose responses (normalized to responses observed from the highest concentration per compound) were observed for the sweet compounds GA-2, saccharin, acesulfame-K, and sucrose in the receptor combination T1R2+3 (Fig 6b), matching the biological thresholds observed in vivo (Fig 6a). Dose responses were not observed for bitter or umami compounds (Fig 6b)