User:LWachhaus

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
unacceptable	MOUSE:TS1R2	2019-03-10 15:06:43 CDT	GO:0033041 sweet taste receptor activity (F)	PMID:11509186	ECO:0001565 cell-based assay evidence	Dose responses (normalized to responses observed from the highest concentration per compound) were observed for the sweet compounds GA-2, saccharin, acesulfame-K, and sucrose in the receptor combination T1R2+3 (Fig 6b), matching the biological thresholds observed in vivo (Fig 6a). Dose responses were not observed for bitter or umami compounds (Fig 6b)	challenge
acceptable	MOUSE:TS1R2	2019-03-24 21:58:33 CDT	GO:0033041 sweet taste receptor activity (F)	PMID:11509186	ECO:0001249 fluorescence evidence used in manual assertion	To demonstrate specificity of the sweet taste receptor, the pathway of G-protein coupled receptors linked to the receptor was modified to increase intracellular calcium which was observed using the fluorescent FURA-2 calcium indicator dye. An increase in cellular calcium was observed (normalized to responses observed from the highest concentration per compound) in the sweet compounds GA-2, saccharin, acesulfame-K, and sucrose in the receptor combination T1R2+3 (Fig 6b), matching the biological thresholds observed in vivo (Fig 6a). Dose responses were not observed for bitter or umami compounds (Fig 6b). As intracellular calcium was not shown to increase upon exposure to any compound other than sugar and artificial sweeteners, the function of T1Rs as a sweet taste receptors is maintained.	challenge

acceptable:1
unacceptable:1
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Annotations challenged by LWachhaus

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	SRana, Team Green B 2019	DEIRA:DDRB	GO:0045002 - double-strand break repair via single-strand annealing (P)	PMID:21968057	ECO:0001141 [3H]-thymidine incorporation assay evidence used in manual assertion	challenge	C: 2
acceptable	CColson, Team Blue A 2019	THET2:SODM	GO:0071793 - bacillithiol biosynthetic process (P)	PMID:30940703	ECO:0001272 high-performance liquid chromatography evidence used in manual assertion	challenge	C: 2

0 annotations fixed by LWachhaus