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DEIRA:DDRB
Contents
| Species (Taxon ID) | Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 /LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422). (243230) | |
| Gene Name(s) | ddrB | |
| Protein Name(s) | Single-stranded DNA-binding protein DdrB
DNA damage response protein B | |
| External Links | ||
| UniProt | Q9RY80 | |
| EMBL | AE000513 BK006794 | |
| PIR | D75563 | |
| RefSeq | NP_293796.1 | |
| PDB | 4HQB | |
| PDBsum | 4HQB | |
| STRING | 243230.DR_0070 | |
| EnsemblBacteria | AAF09667 | |
| GeneID | 1798604 | |
| KEGG | dra:DR_0070 | |
| PATRIC | 21627611 | |
| eggNOG | NOG70958 | |
| HOGENOM | HOG000099548 | |
| OrthoDB | EOG61KBJ8 | |
| BioCyc | DRAD243230:GH46-74-MONOMER | |
| Proteomes | UP000002524 | |
| GO | GO:0003697 GO:0071465 GO:0071480 GO:0006281 | |
| InterPro | IPR024305 | |
| Pfam | PF12747 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0045002 |
double-strand break repair via single-strand annealing |
ECO:0001141 |
[3H]-thymidine incorporation assay evidence used in manual assertion |
P |
In Figure 3 Panel A, the graphs are showing the rate of DNA synthesis in wildtype strain of the bacteria and the strain with the knockout of ddrB gene. Both strains are allowed to grow normally as well as subjected to radiation. They used a [3H]Thymidine incorporation assay to measure rate of DNA synthesis. There was an increased lag phase in DNA synthesis by an hour in the knockout compared to the wildtype strain. This lag was associated with a delay in fragment reassembly shown by PFGE, Panel B. But the rate of DNA synthesis and the fragment reassembly after this lag period was very similar to wildtype. ddrB protein also has high affinity for single stranded DNA and has similar properties to E. coli SSB protein. This is likely indicates that DdrB is involved in a very early step of DNA double strand break repair that occurs prior to strand-annealing and not in annealing the newly synthesized DNA strands itself. |
complete | ||||
|
involved_in |
GO:0071480 |
cellular response to gamma radiation |
ECO:0000270 |
expression pattern evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0071465 |
cellular response to desiccation |
ECO:0000270 |
expression pattern evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006281 |
DNA repair |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003697 |
single-stranded DNA binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
NOT|enables |
GO:0003690 |
double-stranded DNA binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006974 |
cellular response to DNA damage stimulus |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003677 |
DNA binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006281 |
DNA repair |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Bouthier de la Tour, C et al. (2011) The deinococcal DdrB protein is involved in an early step of DNA double strand break repair and in plasmid transformation through its single-strand annealing activity. DNA Repair (Amst.) 10 1223-31 PubMed GONUTS page
- ↑ 2.0 2.1 2.2 Tanaka, M et al. (2004) Analysis of Deinococcus radiodurans's transcriptional response to ionizing radiation and desiccation reveals novel proteins that contribute to extreme radioresistance. Genetics 168 21-33 PubMed GONUTS page
- ↑ 3.0 3.1 Norais, CA et al. (2009) DdrB protein, an alternative Deinococcus radiodurans SSB induced by ionizing radiation. J. Biol. Chem. 284 21402-11 PubMed GONUTS page
