CELJU:B3PDN7

Species (Taxon ID)	Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp.cellulosa). (498211)
Gene Name(s)	cel3B (ECO:0000313 with EMBL:ACE83024.1)
Protein Name(s)	Glucan 1,4-beta-glucosidase, putative, cel3B (ECO:0000313 with EMBL:ACE83024.1)
External Links
UniProt	B3PDN7
EMBL	CP000934
RefSeq	WP_012487127.1
ProteinModelPortal	B3PDN7
STRING	498211.CJA_1497
CAZy	GH3
EnsemblBacteria	ACE83024
KEGG	cja:CJA_1497
eggNOG	ENOG4107QK4 COG1472
HOGENOM	HOG000031214
OMA	PMWVNAE
OrthoDB	419160at2
BioCyc	CJAP498211:G1GB3-1508-MONOMER
Proteomes	UP000001036
GO	GO:0031217 GO:0005975
Gene3D	3.20.20.300 3.40.50.1700
InterPro	IPR002772 IPR036881 IPR001764 IPR036962 IPR017853
Pfam	PF00933 PF01915
PRINTS	PR00133
SUPFAM	SSF51445 SSF52279

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
enables	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001764 InterPro:IPR002772 InterPro:IPR036881 InterPro:IPR036962	F		Seeded From UniProt
involved_in	GO:0005975	carbohydrate metabolic process	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001764 InterPro:IPR002772 InterPro:IPR036881 InterPro:IPR036962	P		Seeded From UniProt
enables	GO:0031217	glucan 1,4-beta-glucosidase activity	GO_REF:0000003	ECO:0000501	evidence used in automatic assertion	EC:3.2.1.74	F		Seeded From UniProt
enables	GO:0016787	hydrolase activity	GO_REF:0000038	ECO:0000323	imported automatically asserted information used in automatic assertion	UniProtKB-KW:KW-0378	F		Seeded From UniProt
involved_in	GO:0008152	metabolic process	GO_REF:0000038	ECO:0000323	imported automatically asserted information used in automatic assertion	UniProtKB-KW:KW-0326	P		Seeded From UniProt
enables	GO:0016798	hydrolase activity, acting on glycosyl bonds	GO_REF:0000038	ECO:0000323	imported automatically asserted information used in automatic assertion	UniProtKB-KW:KW-0326	F		Seeded From UniProt
enables	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001764 InterPro:IPR002772 InterPro:IPR036881 InterPro:IPR036962	F		Seeded From UniProt
involved_in	GO:0005975	carbohydrate metabolic process	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001764 InterPro:IPR002772 InterPro:IPR036881 InterPro:IPR036962	P		Seeded From UniProt
enables	GO:0031217	glucan 1,4-beta-glucosidase activity	GO_REF:0000003	ECO:0000501	evidence used in automatic assertion	EC:3.2.1.74	F		Seeded From UniProt
enables	GO:0016787	hydrolase activity	GO_REF:0000038	ECO:0000323	imported automatically asserted information used in automatic assertion	UniProtKB-KW:KW-0378	F		Seeded From UniProt
involved_in	GO:0008152	metabolic process	GO_REF:0000038	ECO:0000323	imported automatically asserted information used in automatic assertion	UniProtKB-KW:KW-0326	P		Seeded From UniProt
enables	GO:0016798	hydrolase activity, acting on glycosyl bonds	GO_REF:0000038	ECO:0000323	imported automatically asserted information used in automatic assertion	UniProtKB-KW:KW-0326	F		Seeded From UniProt
	GO:2000892	cellobiose catabolic process	PMID:28118504	ECO:0001225	knockout evidence used in manual assertion		P		Mutant strains with individual and combinatorial mutations were generated and growth assays were conducted to determine which genes were essential for allowing Cellvibrio japonicus to metabolize cellobiose, a disaccharide that composes cellulose. According to Figure 1A, cel3B deletion most significantly hinders growth of C. japonicus on cellobiose. Figure 2D also showed that the cel3B single mutants (triangles) had decreased cellobiose consumption and a longer lag phase compared to the wild type (circles). This shows that the gene product of cel3B, an enzyme, helps the cell use cellobiose as a carbon source.	complete CACAO 13660
	GO:2000891	cellobiose metabolic process	PMID:28118504	ECO:0001147	anion-exchange chromatography evidence used in manual assertion		P		In Figure 2, wild type C. japonicus and cel3b, cel3Acel3B, and 4BG mutants were grown on cellobiose and glucose (control). The four strains showed no significant changes in glucose consumption during growth (Fig. 2A & 2B). However, the cel3b mutant showed a decreased rate of cellobiose consumption (Fig. 2C & 2D). This shows that cel3b has a role in efficient cellobiose utilization in C. japonicus.	complete CACAO 13722
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References

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