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Cacao
GO:0015973 | guanosine pentaphosphate biosynthetic process | PMID:30478087 | ECO:0007110 IMP: Inferred from Mutant Phenotype | Biological Process | The authors used a mutant DksA gene to portray the relationship between DksA and the up-regulation of (p)ppGpp levels in starvation-like conditions (Figure 7). | complete | ||
This annotation made on page: BORBU:O51190 By: ABatista (group Team Blue A 2019) on 2019-03-31 18:19:32 CDT. |
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Entry Type | Challenging User,Group | Time/Date | Challenge Reason | Points/Assessment | ||||||||||||||||||||||
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Challenge | CXie, Team Red B 2019 | 2019-04-02 22:36:11 CDT | The note needs to differentiate between the mutant dksA and mutant dKsA pDksA strains. Mutant dksA is significantly different from WT, while dksA pDksA is not. Your current note only mentions mutant dksA compared to WT. It is also important to explain that Fig 7B is normalized to (p)ppGpp + GTP and does not directly represent (p)ppGpp levels, which could be inferred from your current note. | 1 | ||||||||||||||||||||||
Challenge | MGuldan, Team Green A 2019 | 2019-04-01 08:39:14 CDT | I think the note needs more detail and information about the experiment. How does the mutant DksA gene show that relationship? What test was actually done in figure 7? | 2 | ||||||||||||||||||||||
Public Assessment | Ivanerill | 2019-04-18 10:41:13 CDT | No notes given. | Acceptable ✔ Protein ✔ Publication ✔ Qualifier ✔ Go term ✔ Evidence ✔ With/From ✔ Notes ✔ Unique/Original | ||||||||||||||||||||||
Public Assessment | DanielRenfro | 2019-04-17 10:28:17 CDT | This annotation has been flagged because it has been edited since last assessment
| Flagged | ||||||||||||||||||||||
Public Assessment | Ivanerill | 2019-04-08 16:50:56 CDT | You chose a difficult figure for your annotation. The authors do not "demonstrate" that dksA is involved in "guanosine pentaphosphate biosynthetic process", just that it "might" be involved in its regulation. A bit tenuous overall. | Requires Changes ✔ Protein ✔ Publication ✔ Qualifier ✗ Go term ✔ Evidence ✔ With/From ✗ Notes ✔ Unique/Original | ||||||||||||||||||||||
Public Assessment | DanielRenfro | 2019-04-03 09:35:00 CDT | This annotation has been flagged because it has been edited since last assessment
| Flagged |