User:MGuldan

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	ECOLI:SLYA	2019-03-29 18:28:41 CDT	GO:0045893 positive regulation of transcription, DNA-templated (P)	PMID:17892462	ECO:0001204 in vitro transcription assay evidence used in manual assertion	Figure 5 shows the results of an In vitro transcription assay in which 20 ng of linearized pGS1886 DNA was incubated with RNA polymerase (RNAP) and differing amounts of H-NS and SlyA. H-NS is shown to inhibit transcription of hlyE in Figure 5C through use of a PCR-amplified DNA fragment extending from −474 to +222 that includes the hlyE promoter, seemingly completely inhibiting it at H-NS concentration of 4.0 μM. Figure 5D shows that transcription can be restored by increasing concentrations of SlyA through using the same In vitro transcription assay process and 2.0 μM of H-NS. At about 1.0 μM of SlyA, hlyE appears to be transcribed at similar levels to the control, containing no H-NS. From this it can be concluded that H-NS represses hlyE transcription, and SlyA derepresses hlyE transcription. Therefore, SlyA positively regulates transcription of hlyE.	challenge
acceptable	YERPE:A0A384L3J4	2019-04-10 13:52:10 CDT	GO:0045892 negative regulation of transcription, DNA-templated (P)	PMID:19703315	ECO:0005657 primer extension assay evidence used in manual assertion	Figure 5 shows the primer extension analysis of the sycO-ypkA-yopJ operon with and without CRP. A wild type with CRP and a strain without CRP were used. The WT shows no primer extension product while the strain without CRP shows product. The presence of the primer extension product in deleted CRP indicates mRNA presence. The lack of primer extension product in the WT indicates no mRNA presence. Because mRNA presence is high only in the deleted CRP strain, CRP is repressing transcription of the sycO-ypkA-yopJ operon.	challenge

acceptable:2
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by MGuldan

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	ABatista, Team Blue A 2019	BORBU:O51190	GO:0015972 - guanosine pentaphosphate metabolic process (P)	PMID:30478087	ECO:0007110 thin layer chromatography evidence used in manual assertion	challenge	C: 2
acceptable	CColson, Team Blue A 2019	THET2:SODM	GO:0071793 - bacillithiol biosynthetic process (P)	PMID:30940703	ECO:0001272 high-performance liquid chromatography evidence used in manual assertion	challenge	C: 2

0 annotations fixed by MGuldan