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PMID:19703315
| Citation |
Zhan, L, Yang, L, Zhou, L, Li, Y, Gao, H, Guo, Z, Zhang, L, Qin, C, Zhou, D and Yang, R (2009) Direct and negative regulation of the sycO-ypkA-ypoJ operon by cyclic AMP receptor protein (CRP) in Yersinia pestis. BMC Microbiol. 9:178 |
|---|---|
| Abstract |
Pathogenic yersiniae, including Y. pestis, share a type III secretion system (T3SS) that is composed of a secretion machinery, a set of translocation proteins, a control system, and six Yop effector proteins including YpkA and YopJ. The cyclic AMP receptor protein (CRP), a global regulator, was recently found to regulate the laterally acquired genes (pla and pst) in Y. pestis. The regulation of T3SS components by CRP is unknown. |
| Links |
PubMed PMC2738681 Online version:10.1186/1471-2180-9-178 |
| Keywords |
Bacterial Proteins/genetics; Base Sequence; Binding Sites; Cyclic AMP Receptor Protein/genetics; Cyclic AMP Receptor Protein/metabolism; Gene Expression Regulation, Bacterial; Genes, Bacterial; Molecular Sequence Data; Operon; Promoter Regions, Genetic; Protein-Serine-Threonine Kinases/genetics; RNA, Bacterial/genetics; Transcription Initiation Site; Yersinia pestis/genetics; Yersinia pestis/metabolism |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0045892: negative regulation of transcription, DNA-templated |
ECO:0005657: primer extension assay evidence used in manual assertion |
P |
Figure 5 shows the primer extension analysis of the sycO-ypkA-yopJ operon with and without CRP. A wild type with CRP and a strain without CRP were used. The WT shows no primer extension product while the strain without CRP shows product. The presence of the primer extension product in deleted CRP indicates mRNA presence. The lack of primer extension product in the WT indicates no mRNA presence. Because mRNA presence is high only in the deleted CRP strain, CRP is repressing transcription of the sycO-ypkA-yopJ operon. |
complete | ||||
Notes
See also
References
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