User:Shireen2

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
unacceptable	9CAUD:A0A0K2D0I8	2016-04-04 09:02:30 CDT	GO:0004309 exopolyphosphatase activity (F)	PMID:24275100	ECO:0000315 mutant phenotype evidence used in manual assertion	there is an evidence that Exopolyphosphat of Pseudomonas aeruginosa is essential for flagellum dependent swimming and swarming motility. the author evaluated a WT,DppX and DppxC strain on LB medium plate and incubated at 37uC for 24h. the swimming motility of Dppx was 55-75%less than WT. the Dppx strain also decrease the swarming motility: 65-80% with respect to WT.	challenge
unacceptable	9CAUD:A0A0K2D0I8	2016-04-04 08:47:41 CDT	GO:0004309 exopolyphosphatase activity (F)	PMID:24569519	ECO:0000315 mutant phenotype evidence used in manual assertion	to test the motility and biofilm formation of C.jejuni delta ppx mutants, the author assessed the motility on 0.4% soft agar at 42C for 24h. motility was quantified by measuring the swarming zone around the stabbed area. the result indicate that deleting the gene ppx in C.jejuni affect the amount of biofilm, therefore its affect the motility. also, ppx/gppa genes play a role in transmission related phenotypes such as motility and biofilm formation in C.jejuni.	challenge
unacceptable	9CAUD:A0A0K2D0I8	2016-04-04 08:54:38 CDT	GO:0004309 exopolyphosphatase activity (F)	PMID:24569519	ECO:0000317 genomic context evidence used in manual assertion	the phylogenetic analysis using MEGA-5 showed that C. jejuni PPX2 clustered with E.coli and mycobacterium tuberculosis species. (fig.S1) also HHpred show that gene TSARBOMBA_148 homologues to Mycobacterium tuberculosis with e-value= 8.8e-28 and probable 99.96.	challenge
updatedbyinstructor	CAMJJ:A0A0H3PES2	2016-04-06 11:05:18 CDT	GO:0071978 bacterial-type flagellum-dependent swarming motility (P)	PMID:24569519	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4. Motility and biofilm formation of C. jejuni ∆ppx mutants. (A) Motility was assessed on 0.4% soft agar at 42 °C for 24 h. (B) Motility was quantified by measuring the swarming zone (diameter in cm) surrounding the stabbed area. Each bar represents the average from 3 independent experiments with duplicate samples. *P ≤ 0.05 **P ≤ 0.001.	challenge
acceptable	BACCE:A0A0K6LY49	2016-04-06 05:53:28 CDT	GO:0071977 bacterial-type flagellum-dependent swimming motility (P)	PMID:15572452	ECO:0000315 mutant phenotype evidence used in manual assertion	"plate were inoculated with bacteria from an overnight LB culture on agar plates and incubated at 30C for 8-10h for swimming assays and 12-16h for swarming assay '. The result indicate that the mutants ppx in B. Cereus impaired with the flagella motility	challenge
unacceptable	PSEAE:PPX	2016-04-11 09:37:56 CDT	GO:0004309 exopolyphosphatase activity (F)	PMID:24275100	ECO:0000315 mutant phenotype evidence used in manual assertion	there is an evidence that Exopolyphosphat of Pseudomonas aeruginosa is essential for flagellum dependent swimming and swarming motility. the author evaluated a WT,DppX and DppxC strain on LB medium plate and incubated at 37uC for 24h. the swimming motility of Dppx was 55-75%less than WT. the Dppx strain also decrease the swarming motility: 65-80% with respect to WT.	challenge

acceptable:1
unacceptable:4
requires_changes:0
flagged:0

Annotations challenged by Shireen2

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
unacceptable	Natjack1, Team Green B	9CAUD:A0A0K2CZZ1	GO:0004604 - phosphoadenylyl-sulfate reductase (thioredoxin) activity (F)	GO_REF:0000100	ECO:0000250 sequence similarity evidence used in manual assertion	challenge	C: 3
unacceptable	E63, Team Blue A	9CAUD:A0A0K2D000	GO:0050661 - NADP binding (F)	PMID:6815179	ECO:0000250 sequence similarity evidence used in manual assertion	challenge	C: 3
unacceptable	E63, Team Blue A	9CAUD:A0A0K2D000	GO:0004146 - dihydrofolate reductase activity (F)	PMID:26147643	ECO:0000250 sequence similarity evidence used in manual assertion	challenge	C: 4
unacceptable	Shireen2, Team Red A	9CAUD:A0A0K2D0I8	GO:0004309 - exopolyphosphatase activity (F)	PMID:24275100	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 3
unacceptable	Shireen2, Team Red A	9CAUD:A0A0K2D0I8	GO:0004309 - exopolyphosphatase activity (F)	PMID:24569519	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 2

0 annotations fixed by Shireen2

What do the icons mean in the status column?