User:Shahrk

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
flagged	BPT4:HOLIN	2017-04-26 07:44:59 CDT	GO:0019835 cytolysis (P)	PMID:22389108	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2, plots a graph with A550 on the y axis and time of induction (min) on the x axis. The graph illustrated how the alleles tQ87am and tQ209am contain partial Holin activity. When the two alleles are present in the sample; the turbidity decrease indicating the bacteria has died, due to lysis activity. The alleles tQ87am and tQ209am supports gradual lysis after 40 min and 75 min, respectively.	challenge
flagged	BACSU:XHLB	2017-04-28 14:14:07 CDT	GO:0019835 cytolysis (P)	PMID:9555893	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 6, contains a graph measuring the OD550 (optical density) over time (hours). The lines on the graph showed various lysis genes. These genes had a combination of mutant strains of xhylA (encodes for putative endolysin), xhlB (encodes for putative holin), or xlyA inject inside of them. According to the graph the lines with with solid triangles (POP1234), solid squares (SK109), and a hallow square (SK110) all decreased in turbidity. Since three of these cultures contained contained strains of either xlyA or xhlB; indicating the bacteria in the culture died due to lysis. This proves holin and endolysin play a major role in lysis.	challenge

acceptable:0
unacceptable:0
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flagged:2

Annotations challenged by Shahrk

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
unacceptable	Ig4, Team Green-A	9VIRU:A0A1B0VMI2	GO:0016787 - hydrolase activity (F)	PMID:4936722	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 3
flagged	Tosin, Team EKSUtopaz	BPKVM:Q6WHP0	GO:0009435 - NAD biosynthetic process (P)	PMID:28167526	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1

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