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Cacao

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GO:0009435NAD biosynthetic processPMID:28167526IDA: Inferred from Direct Assay P
This annotation made on page: BPKVM:Q6WHP0
By: Tosin (group Team EKSUtopaz) on 2017-04-11 03:22:01 CDT.



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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeShahrk,
?		2017-04-12 10:09:49 CDT

To strengthen the annotation, explain the y and x axis of the graph and what each symbol/line represents. Also describe how the variables (x and y axis) influence the appearance of the graph. For example, explain how the lines with and without pZL405nadV+ are different. Overall, it's unclear what the figure displays.

None, yet.
Public
Assessment		DanielRenfro2017-04-16 03:17:25 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0009435 NAD biosynthetic process PMID:28167526 IDA: Inferred from Direct Assay P Figure 3 and 5show ATP activity and complementation of Samonella Typhimurium pyridine nucleotide auxotroph by the nadV+clone (pZL405nadV+)

Figure 7 (explanation from the manuscript).

Figure Title: Substrate specificity of the KVP40 NatV Nudix pyrophosphatase.

A) Activity was measured in the phosphate release assay using 1.5 mM of substrate and 50 pmole NatV-His6 present in reactions incubated at 37°C. Values shown are averages from two assays. Stoichiometry of phosphate per substrate is 1:1.


B) Mass spectrometry analysis of KVP40 NatV 754 Nudix hydrolase substrate specificity. Specific activity was obtained with 1.5 mM substrate and 1 μg NatV, incubated at 37°C. Values are averages from duplicate assays (triplicate for ADP-ribose). The specific activity on ADP-ribose was 0.6 μmole AMP sec^-1 µg^-1 NatV-His6.

complete
CACAO 12596
on BPKVM:Q6WHP0
Flagged


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