It is now the 5th OPEN Period for CACAO Fall 2017! It will end on Tuesday November 21, 2017 at 11:59 pm CST
This is your chance to make annotations OR challenge other team's annotations. You may also DEFEND or suggest improvements to your own annotations IF they have been challenged. Please note, although we ENCOURAGE challenges, an excess of identical challenges that do not appear to be applicable to the annotation or well thought out will be considered spam and ignored.

Have any questions? Please email us at

Category:Team EKSUtopaz

Jump to: navigation, search
StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
flagged9CAUD:A8D3R5Tosin, Team EKSUtopaz2017-04-11 02:34:08 CDTGO:0008233 peptidase activity (F)PMID:28357414ISA with/from UniProtKB:KW-0645

figure S2 shows that SAP-2_gp10 is a homologue of Andhra_gp10(confirmed by a BLASTP of Andhra_gp10).

Figure 2 shows that the wildtype Andhra_gp10 is a peptidase with a CHAP(cysteine, histidine-dependent amidohydrolases/peptidases) domain and mutations of the cysteine(C354A) and histidine (H420A) in the CHAP domain abrogate the peptidase activity of Andhra_gp10. Currently there is no UNIPROT ID for Andhra_gp10. Hence, its Genbank Accession Number(AQT27832) has been included in the with/from field.

BPKVM:Q6WHZ2Tosin, Team EKSUtopaz2017-04-11 03:07:35 CDTGO:0000309 nicotinamide-nucleotide adenylyltransferase activity (F)PMID:28167526IDA

Figure 7 shows substrate specificity of NatV Nudix hydrolase as measured by Pi released from the respective substrate. At 37 degree centigrade, the preferred substrate are ADP-ribose, NAD+ and NADH with little differences in activity between them.

The stoichiometry of Pi from all of these Nudix hydrolase reactions is 1:1, most yielding an AMP. In reactions where AMP could be directly measured using mass spectrophotometer, ADP-ribose was also preferred substrate, with NAD+, NADH, with FAD showing reactivity but not at a lower rate

flaggedBPKVM:Q6WHP0Tosin, Team EKSUtopaz2017-04-11 03:22:01 CDTGO:0009435 NAD biosynthetic process (P)PMID:28167526IDA

Figure 3 and 5show ATP activity and complementation of Samonella Typhimurium pyridine nucleotide auxotroph by the nadV+clone (pZL405nadV+)

Figure 7 (explanation from the manuscript).

Figure Title: Substrate specificity of the KVP40 NatV Nudix pyrophosphatase.

A) Activity was measured in the phosphate release assay using 1.5 mM of substrate and

50 pmole NatV-His6 present in reactions incubated at 37°C. Values shown are averages from two assays. Stoichiometry of phosphate per substrate is 1:1.

B) Mass spectrometry analysis of KVP40 NatV 754 Nudix hydrolase substrate specificity. Specific activity was obtained with 1.5 mM substrate and 1 μg NatV, incubated at 37°C. Values are averages from duplicate assays (triplicate for ADP-ribose). The specific activity on ADP-ribose was 0.6 μmole AMP sec^-1 µg^-1 NatV-His6.

9CAUD:B7T0K1Tosin, Team EKSUtopaz2017-04-22 19:27:45 CDTGO:0003796 lysozyme activity (F)PMID:22729533IDA

FIGURE 1 shows that the lysozyme-like domain (TG1) of Tape Measure Protein (TMP) of Staphylococcus aureus bacteriophage vB_SauS-phiIPLA35 has peptidoglycan muramidase activity and is most likely a muramidase that can digest MurNAc-GlcNAc linkages.

The lytic activity TG1 protein against S. aureus is shown in Figure 2.

BPMR1:A7VMY9Tosin, Team EKSUtopaz2017-04-22 19:37:20 CDTGO:0003796 lysozyme activity (F)PMID:18462391IDA

Figure 2 and Table 1 show that gp61 has lytic activity against S. aureus.

It also demostrates that this activity is localized to two domains (amino acids 1-150 and 401-624) of the polypeptide. Figure 4 shows that gp61 is located at the tail tip.


What do the icons mean in the status column?

assessment icons.jpg

Pages in category "Team EKSUtopaz"

The following 3 pages are in this category, out of 3 total.

Jump to pages starting with: D K T