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BPKVM:Q6WHP0

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Species (Taxon ID) Vibrio phage KVP40 (isolate Vibrioparahaemolyticus/Japan/Matsuzaki/1991) (KVP40) (Bacteriophage KVP40). (1283340)
Gene Name(s) nadV (ECO:0000313 with EMBL:AAQ64333.1)
Protein Name(s) Nicotinamide phosphoribosyl transferase (ECO:0000313 with EMBL:AAQ64333.1)
External Links
UniProt Q6WHP0
EMBL AY283928
RefSeq NP_899510.1
ProteinModelPortal Q6WHP0
GeneID 2546058
KEGG vg:2546058
KO K03462
OrthoDB VOG090000WA
Proteomes UP000001785
GO GO:0004514
GO:0009435
CDD cd01569
InterPro IPR007229
IPR016471
IPR002638
PANTHER PTHR43816:SF1
Pfam PF04095
PIRSF PIRSF005943
SUPFAM SSF51690

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0009435

NAD biosynthetic process

PMID:28167526[1]

ECO:0000314

P

Figure 3 and 5show ATP activity and complementation of Samonella Typhimurium pyridine nucleotide auxotroph by the nadV+clone (pZL405nadV+) Figure 7 (explanation from the manuscript).

Figure Title: Substrate specificity of the KVP40 NatV Nudix pyrophosphatase.

A) Activity was measured in the phosphate release assay using 1.5 mM of substrate and 50 pmole NatV-His6 present in reactions incubated at 37°C. Values shown are averages from two assays. Stoichiometry of phosphate per substrate is 1:1.


B) Mass spectrometry analysis of KVP40 NatV 754 Nudix hydrolase substrate specificity. Specific activity was obtained with 1.5 mM substrate and 1 μg NatV, incubated at 37°C. Values are averages from duplicate assays (triplicate for ADP-ribose). The specific activity on ADP-ribose was 0.6 μmole AMP sec^-1 µg^-1 NatV-His6.

complete
CACAO 12596

enables

GO:0003824

catalytic activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR013785

F

Seeded From UniProt

complete

enables

GO:0004514

nicotinate-nucleotide diphosphorylase (carboxylating) activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR036068

F

Seeded From UniProt

complete

involved_in

GO:0009435

NAD biosynthetic process

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR036068

P

Seeded From UniProt

complete

enables

GO:0016740

transferase activity

GO_REF:0000038

ECO:0000323

imported automatically asserted information used in automatic assertion

UniProtKB-KW:KW-0808

F

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. Lee, JY et al. (2017) Vibrio phage KVP40 Encodes a Functional NAD+ Salvage Pathway. J. Bacteriol. PubMed GONUTS page