|Status||Page||User||Date/Time||GO Term (Aspect)||Reference||Evidence||Notes||Links|
|9VIRU:A0A1B0VMI2||Ig4, Team Green-A||2017-04-09 14:56:34 CDT||GO:0016787 hydrolase activity (F)||PMID:4936722||IDA|
Evidence code IPI under IDA. To confirm that the AH14a_p93 gene encodes functional peptidoglycan hydrolase enzyme, the gene was cloned in E. coli under control of a promoter in Fig 6. Cell lysis occurred when the putative hydrolyse gene was induced by IPTG. A lethal effect was observed on the heterological host. Under Standard Molecular Biological Procedures a Restriction digestion assay was performed to digest the PCR products with a restriction enzyme.
|BPT4:ENLYS||Quocbui1, Team Green-A||2017-04-09 15:13:56 CDT||GO:0003796 lysozyme activity (F)||PMID:16885461||IDA|
Gaidelyte et al. found that the endolysin was unstable and degraded in crude E. coli cell extracts causing a smear on the zymogram, and also found that the endolysin could function independently of the presence of divalent metal cations. The smear on the zymogram is indicative of selectivity of endolysin on peptidoglycan from different hosts.
|BACTU:J3UKZ5||Quocbui1, Team Green-A||2017-04-09 15:42:56 CDT||GO:0019835 cytolysis (P)||PMID:16103125||IDA|
Fig. 4A-D demonstrates that the protein has lytic activity by observing percent absorption or time (second) and that in some species the catalytic domain alone yields higher activity than the full length protein while for other species it is the opposite.
|9CAUD:Q9G097||Ma16, Team Green-A||2017-04-09 16:10:10 CDT||GO:0098004 virus tail fiber assembly (P)||PMID:27824135||IMP|
56 mutants were created to characterize which domains of TP901-1's tape measure gene (TMP) were essential for infection of the phage and it's tail assembly. Through this experiment, TP901-1's N-terminal 154 amino acid residues, C-terminal 60 residues, and hydrophobic region were revealed to be required for TP901's infectivity. This is shown in fig.2 in the paper, where removing 30 amino acids from both N and C-termini cause phage assembly to be aberrant, while removal of the hydrophobic region causes tai length reduction.
|BPSPP:O48490||Quocbui1, Team Green-A||2017-04-09 17:07:23 CDT||GO:0004527 exonuclease activity (F)||PMID:12670970||IDA|
Figure 4: Fluorescent signal on dsDNA has a higher intensity than on ssDNA or on nucleotides. The decrease in fluorescent signal is indicative of DNA digestion.
|9VIRU:Q6X3T9||Quocbui1, Team Green-A||2017-04-23 14:47:50 CDT||GO:0061783 peptidoglycan muralytic activity (F)||PMID:16885461||IDA|
Figure 4 shows the zymogram where E. coli cell extracts are degraded. Furthermore, the smear on the zymogram is indicative of selectivity of peptidoglycan from different hosts.
|9VIRU:D0R7H8||Quocbui1, Team Green-A||2017-04-23 17:46:11 CDT||GO:0019835 cytolysis (P)||PMID:26085488||IDA|
Figure 3 reveals that the protein activity results in clear zones of the bacterial lawn.
|9VIRU:D0R7I3||Quocbui1, Team Green-A||2017-04-23 17:55:29 CDT||GO:0019835 cytolysis (P)||PMID:26085488||IDA|
Figure 4 shows the lytic activity against different hosts. It shows that the protein has specificity against P. polymyxa over other Paenibacillus bacteria.