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PMID:16885461
| Citation |
Gaidelyte, A, Cvirkaite-Krupovic, V, Daugelavicius, R, Bamford, JK and Bamford, DH (2006) The entry mechanism of membrane-containing phage Bam35 infecting Bacillus thuringiensis. J. Bacteriol. 188:5925-34 |
|---|---|
| Abstract |
The temperate double-stranded DNA bacteriophage Bam35 infects gram-positive Bacillus thuringiensis cells. Bam35 has an icosahedral protein coat surrounding the viral membrane that encloses the linear 15-kbp DNA genome. The protein coat of Bam35 uses the same assembly principle as that of PRD1, a lytic bacteriophage infecting gram-negative hosts. In this study, we dissected the process of Bam35 entry into discrete steps: receptor binding, peptidoglycan penetration, and interaction with the plasma membrane (PM). Bam35 very rapidly adsorbs to the cell surface, and N-acetyl-muramic acid is essential for Bam35 binding. Zymogram analysis demonstrated that peptidoglycan-hydrolyzing activity is associated with the Bam35 virion. We showed that the penetration of Bam35 through the PM is a divalent-cation-dependent process, whereas adsorption and peptidoglycan digestion are not. |
| Links |
PubMed PMC1540063 Online version:10.1128/JB.00107-06 |
| Keywords |
Bacillus Phages/metabolism; Bacillus thuringiensis/virology; Cell Membrane/virology; Endopeptidases/metabolism; Muramic Acids/chemistry; Receptors, Virus/chemistry; Receptors, Virus/physiology |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0003796: lysozyme activity |
ECO:0000314: |
F |
Gaidelyte et al. found that the endolysin was unstable and degraded in crude E. coli cell extracts causing a smear on the zymogram, and also found that the endolysin could function independently of the presence of divalent metal cations. The smear on the zymogram is indicative of selectivity of endolysin on peptidoglycan from different hosts. |
complete | ||||
| GO:0061783: peptidoglycan muralytic activity |
ECO:0000314: |
F |
Figure 4 shows the zymogram where E. coli cell extracts are degraded. Furthermore, the smear on the zymogram is indicative of selectivity of peptidoglycan from different hosts. |
complete | ||||
Notes
See also
References
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