User:Quocbui1

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
unacceptable	BPT4:ENLYS	2017-04-09 15:13:56 CDT	GO:0003796 lysozyme activity (F)	PMID:16885461	ECO:0000314 direct assay evidence used in manual assertion	Gaidelyte et al. found that the endolysin was unstable and degraded in crude E. coli cell extracts causing a smear on the zymogram, and also found that the endolysin could function independently of the presence of divalent metal cations. The smear on the zymogram is indicative of selectivity of endolysin on peptidoglycan from different hosts.	challenge
unacceptable	BACTU:J3UKZ5	2017-04-09 15:42:56 CDT	GO:0019835 cytolysis (P)	PMID:16103125	ECO:0000314 direct assay evidence used in manual assertion	Fig. 4A-D demonstrates that the protein has lytic activity by observing percent absorption or time (second) and that in some species the catalytic domain alone yields higher activity than the full length protein while for other species it is the opposite.	challenge
acceptable	BPSPP:O48490	2017-04-09 17:07:23 CDT	GO:0004527 exonuclease activity (F)	PMID:12670970	ECO:0000314 direct assay evidence used in manual assertion	Figure 4: Fluorescent signal on dsDNA has a higher intensity than on ssDNA or on nucleotides. The decrease in fluorescent signal is indicative of DNA digestion.	challenge
updatedbyinstructor	9VIRU:Q6X3T9	2017-04-23 14:47:50 CDT	GO:0061783 peptidoglycan muralytic activity (F)	PMID:16885461	ECO:0000314 direct assay evidence used in manual assertion	Figure 4 shows the zymogram where E. coli cell extracts are degraded. Furthermore, the smear on the zymogram is indicative of selectivity of peptidoglycan from different hosts.	challenge
updatedbyinstructor	9VIRU:D0R7H8	2017-04-23 17:46:11 CDT	GO:0019835 cytolysis (P)	PMID:26085488	ECO:0000314 direct assay evidence used in manual assertion	Figure 3 reveals that the protein activity results in clear zones of the bacterial lawn.	challenge
unacceptable	9VIRU:D0R7I3	2017-04-23 17:55:29 CDT	GO:0019835 cytolysis (P)	PMID:26085488	ECO:0000314 direct assay evidence used in manual assertion	Figure 4 shows the lytic activity against different hosts. It shows that the protein has specificity against P. polymyxa over other Paenibacillus bacteria.	challenge

acceptable:1
unacceptable:3
requires_changes:0
flagged:0

Annotations challenged by Quocbui1

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

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