User:Rizzomi2

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	LISMO:Q8Y783	2014-04-01 15:06:21 CDT	GO:0035635 entry of bacterium into host cell (P)	PMID:24572033	ECO:0000314 direct assay evidence used in manual assertion	Figure 4C: When Listera innocua is supplemented with the gene, Lmo1413, it gains the ability to invade non-phagocytic eukaryotic cells at high level similar to that of Listeria monocytogenes.	challenge
updatedbyinstructor	HELP3:D7FF05	2014-04-01 20:51:44 CDT	GO:0019836 hemolysis by symbiont of host erythrocytes (P)	PMID:24434145	ECO:0000314 direct assay evidence used in manual assertion	Figure 3A: Purified TlyA displayed concentration-dependent hemolytic activity against human erythrocytes. The higher the concentration of TlyA added, the higher the percentage of hemolysis.	challenge
updatedbyinstructor	PSESY:S3ML65	2014-04-01 21:17:51 CDT	GO:0008233 peptidase activity (F)	PMID:24654978	ECO:0000314 direct assay evidence used in manual assertion	Figure 2: When the purified AprA protein was present with flagellin monomers, proteolysis of the flagellin monomers occurred.	challenge
updatedbyinstructor	PSESY:S3MID4	2014-04-01 21:34:58 CDT	GO:0030414 peptidase inhibitor activity (F)	PMID:24654978	ECO:0000314 direct assay evidence used in manual assertion	Figure 2: When the AprA protein was present with flagellin monomers, proteolysis of the flagellin monomers occurred. When the AprI protein was added to the AprA protein/flagellin monomer mixture, proteolysis of the flagellin monomers did not occur. This is showing that aprI is acting as an inhibitor to the aprA gene.	challenge
updatedbyinstructor	LACRE:A0A2R7DXN8	2014-04-02 10:20:08 CDT	GO:0052562 negative regulation by symbiont of host immune response (P)	PMID:24488273	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4A: Compared to the wild type strain of Lactobacillus reuteri, the mutant strain had more TNF present after the assay. This is showing that the mutant failed to suppress TNF production from THP-1 cells.	challenge
updatedbyinstructor	STRAG:A0A1A9E0H6	2014-04-02 11:45:36 CDT	GO:0044651 adhesion of symbiont to host epithelial cell (P)	PMID:24343649	ECO:0000314 direct assay evidence used in manual assertion	Figure 4: When introducing Sal0825 into Lactobacillus lactis, L. lactis displayed a higher adherence rate to host epithelial cells.	challenge
updatedbyinstructor	STRAG:A0A1A9E0H6	2014-04-02 11:51:36 CDT	GO:0007160 cell-matrix adhesion (P)	PMID:24343649	ECO:0000314 direct assay evidence used in manual assertion	Figure 4: When sal0825 was introduced into Lactobacillus lactis, its adherence to immobilized extracellular matrix proteins (fibronectin and laminin) increased significantly.	challenge
updatedbyinstructor	CAMJE:Q0PBN7	2014-04-03 09:48:01 CDT	GO:0044650 adhesion of symbiont to host cell (P)	PMID:24303031	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3A: When the cj0268c was knocked out in Campylobacter jejuni, the adherence to Caco2 cells decreased significantly. When the gene was introduced back into the mutant, the adherence increased back up to wild-type levels. Figure 4B: When the cj0268c gene was introduced into E. coli, the adherence to Caco2 cells increased significantly.	challenge
updatedbyinstructor	9ACTN:U6C6H1	2014-04-03 19:58:37 CDT	GO:0047390 glycerophosphocholine cholinephosphodiesterase activity (F)	PMID:24211038	ECO:0000314 direct assay evidence used in manual assertion	Figure 3 is showing the steady-state kinetics of GPC hydrolysis by GPC-CP.	challenge

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unacceptable:0
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Annotations challenged by Rizzomi2

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
updatedbyinstructor	Rizzomi2, MichSt14A 44	LISMO:Q8Y783	GO:0035635 - entry of bacterium into host cell (P)	PMID:24572033	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2 fixed by Rizzomi2
updatedbyinstructor	Min.lorna, Team Ood	LACRD:A5VK67	GO:0046775 - obsolete suppression by virus of host cytokine production (P)	PMID:24123819	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 2
unacceptable	Min.lorna, Team Ood	STAA8:ARLS	GO:0043709 - cell adhesion involved in single-species biofilm formation (P)	PMID:10869073	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1
unacceptable	Min.lorna, Team Ood	LACRE:E9RM36	GO:0050727 - regulation of inflammatory response (P)	PMID:24123819		challenge	C: 1

1 annotations fixed by Rizzomi2

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