PMID:24303031

Citation	Tareen, AM, Lüder, CG, Zautner, AE, Groβ, U, Heimesaat, MM, Bereswill, S and Lugert, R (2013) The Campylobacter jejuni Cj0268c protein is required for adhesion and invasion in vitro. PLoS ONE 8:e81069
Abstract	Adherence of Campylobacter jejuni to its particular host cells is mediated by several pathogen proteins. We screened a transposon-based mutant library of C. jejuni in order to identify clones with an invasion deficient phenotype towards Caco2 cells and detected a mutant with the transposon insertion in gene cj0268c. In vitro characterization of a generated non-random mutant, the mutant complemented with an intact copy of cj0268c and parental strain NCTC 11168 confirmed the relevance of Cj0268c in the invasion process, in particular regarding adherence to host cells. Whereas Cj0268c does not impact autoagglutination or motility of C. jejuni, heterologous expression in E. coli strain DH5α enhanced the potential of the complemented E. coli strain to adhere to Caco2 cells significantly and, thus, indicates that Cj0268c does not need to interact with other C. jejuni proteins to develop its adherence-mediating phenotype. Flow cytometric measurements of E. coli expressing Cj0268c indicate a localization of the protein in the periplasmic space with no access of its C-terminus to the bacterial surface. Since a respective knockout mutant possesses clearly reduced resistance to Triton X-100 treatment, Cj0268c contributes to the stability of the bacterial cell wall. Finally, we could show that the presence of cj0268c seems to be ubiquitous in isolates of C. jejuni and does not correlate with specific clonal groups regarding pathogenicity or pathogen metabolism.
Links	PubMed PMC3841222 Online version:10.1371/journal.pone.0081069
Keywords
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
CAMJE:Q0PBN7		GO:0044650: adhesion of symbiont to host cell	ECO:0000315:		P		Figure 3A: When the cj0268c was knocked out in Campylobacter jejuni, the adherence to Caco2 cells decreased significantly. When the gene was introduced back into the mutant, the adherence increased back up to wild-type levels. Figure 4B: When the cj0268c gene was introduced into E. coli, the adherence to Caco2 cells increased significantly.	complete CACAO 9751
CAMJE:Q0PBN7	involved_in	GO:0044650: adhesion of symbiont to host cell	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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