PMID:24343649

Citation	Jiang, S and Wessels, MR (2014) BsaB, a novel adherence factor of group B Streptococcus. Infect. Immun. 82:1007-16
Abstract	Streptococcus agalactiae (group B Streptococcus [GBS]) is a leading cause of neonatal sepsis and meningitis, peripartum infections in women, and invasive infections in chronically ill or elderly individuals. GBS can be isolated from the gastrointestinal or genital tracts of up to 30% of healthy adults, and infection is thought to arise from invasion from a colonized mucosal site. Accordingly, bacterial surface components that mediate attachment of GBS to host cells or the extracellular matrix represent key factors in the colonization and infection of the human host. We identified a conserved GBS gene of unknown function that was predicted to encode a cell wall-anchored surface protein. Deletion of the gene and a cotranscribed upstream open reading frame (ORF) in GBS strain 515 reduced bacterial adherence to VK2 vaginal epithelial cells in vitro and reduced GBS binding to fibronectin-coated microtiter wells. Expression of the gene product in Lactococcus lactis conferred the ability to adhere to VK2 cells, to fibronectin and laminin, and to fibronectin-coated ME-180 cervical epithelial cells. Expression of the recombinant protein in L. lactis also markedly increased biofilm formation. The adherence function of the protein, named bacterial surface adhesin of GBS (BsaB), depended both on a central BID1 domain found in bacterial intimin-like proteins and on the C-terminal portion of the BsaB protein. Expression of BsaB in GBS, like that of several other adhesins, was regulated by the CsrRS two-component system. We conclude that BsaB represents a newly identified adhesin that participates in GBS attachment to epithelial cells and the extracellular matrix.
Links	PubMed Online version:10.1128/IAI.01014-13
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
STRAG:A0A1A9E0H6		GO:0044651: adhesion of symbiont to host epithelial cell	ECO:0000314:		P		Figure 4: When introducing Sal0825 into Lactobacillus lactis, L. lactis displayed a higher adherence rate to host epithelial cells.	complete CACAO 9729
STRAG:A0A1A9E0H6		GO:0007160: cell-matrix adhesion	ECO:0000314: direct assay evidence used in manual assertion		P		Figure 4: When sal0825 was introduced into Lactobacillus lactis, its adherence to immobilized extracellular matrix proteins (fibronectin and laminin) increased significantly.	complete CACAO 9730

References

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