Category:MichSt14A 44

Figure 3 is showing the steady-state kinetics of GPC hydrolysis by GPC-CP.

Figure 3A: When the cj0268c was knocked out in Campylobacter jejuni, the adherence to Caco2 cells decreased significantly. When the gene was introduced back into the mutant, the adherence increased back up to wild-type levels.

Figure 4B: When the cj0268c gene was introduced into E. coli, the adherence to Caco2 cells increased significantly.

Figure 4: When sal0825 was introduced into Lactobacillus lactis, its adherence to immobilized extracellular matrix proteins (fibronectin and laminin) increased significantly.

Figure 4: When introducing Sal0825 into Lactobacillus lactis, L. lactis displayed a higher adherence rate to host epithelial cells.

Figure 4A: Compared to the wild type strain of Lactobacillus reuteri, the mutant strain had more TNF present after the assay. This is showing that the mutant failed to suppress TNF production from THP-1 cells.

Figure 2: When the AprA protein was present with flagellin monomers, proteolysis of the flagellin monomers occurred. When the AprI protein was added to the AprA protein/flagellin monomer mixture, proteolysis of the flagellin monomers did not occur. This is showing that aprI is acting as an inhibitor to the aprA gene.

Figure 2: When the purified AprA protein was present with flagellin monomers, proteolysis of the flagellin monomers occurred.

Figure 3A: Purified TlyA displayed concentration-dependent hemolytic activity against human erythrocytes. The higher the concentration of TlyA added, the higher the percentage of hemolysis.

Figure 4C: When Listera innocua is supplemented with the gene, Lmo1413, it gains the ability to invade non-phagocytic eukaryotic cells at high level similar to that of Listeria monocytogenes.