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User:Pclarence

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UMBC Phage Hunters Spring 2015

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptable9CAUD:S5YPI02015-04-08 09:18:30 CDTGO:0006268 DNA unwinding involved in DNA replication (P)other:PMC3729639ECO:0000314 direct assay evidence used in manual assertion

Even though UvsW acts as a Helicase in T4-bacteriophage, it requires the help of another protein gp32. gp32 is a DNA-binding protein, when bounded to the unwinded DNA, enhances the conversion of D-loop structures into unwinded structures. Table1b, lanes 7-12 contains D-loop radioactively labeled dsDNA covered with 2uM of gp32 proteins and exposed to UvsW protein at different time points. Compared to the lanes 1-6, which do no contain gp32 proteins, D-loop structures were relieved at a faster rate in the presence of gp32 proteins.

challenge

acceptable:0
unacceptable:1
requires_changes:0
flagged:0

Annotations challenged by Pclarence

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorKelizabeth,
TeamBlue
BPP2:BPJGO:0098025 - virus tail, baseplate (C)PMID:7483254ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2
acceptableKelizabeth,
TeamBlue
BPT4:TYSYGO:0004799 - thymidylate synthase activity (F)PMID:894793ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
acceptableKelizabeth,
TeamBlue
9CAUD:S5YCV5GO:0004799 - thymidylate synthase activity (F)GO_REF:0000100ECO:0000247 sequence alignment evidence used in manual assertionchallengeC: 1
acceptablePbrittnie,
TeamBlue
BPA51:Q37916GO:0098027 - virus tail, sheath (C)PMID:24673724ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2
updatedbyinstructorDnicholas,
TeamBlue
9CAUD:J9PU17GO:0008745 - N-acetylmuramoyl-L-alanine amidase activity (F)PMID:22524239ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
updatedbyinstructorWmimi,
TeamBlue
9CAUD:S5Y0L8GO:0098027 - virus tail, sheath (C)GO_REF:0000100ECO:0000247 sequence alignment evidence used in manual assertionchallengeC: 3

0 annotations fixed by Pclarence

What do the icons mean in the status column?

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