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User:Pbrittnie

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UMBC Phage Hunters Spring 2015

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableBPA51:Q379162015-04-07 15:33:39 CDTGO:0098027 virus tail, sheath (C)PMID:24673724ECO:0000314 direct assay evidence used in manual assertion

The author's exposed Listeria Bacteriophage A511 baseplate to a-Gp proteins, purified antibodies for cross-linking (with Staphylococcus ISP), and immunogold labeling. The results were found by use of transmission electron micrograph.

In Figure 7, TEM images depict the antibody cross-linking of A511 and ISP. Immunogold labelling is seen as well by black dots on the image. A contracted tail fiber was identified in the A511 tail through the identification of Gp108.

In Figure 8, an immunogold label TEM shows the contracted tail of A511. In addition to antibody cross-linking and in silico prediction, a model of the A511 tail was generated and compared to the TEM image. The presumed location of gene products including tsh (tail sheath protein) are seen in the model.

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Annotations challenged by Pbrittnie

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BPA51:Q37916GO:0098027 - virus tail, sheath (C)PMID:24673724ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2

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