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9CAUD:J9PU17
Contents
| Species (Taxon ID) | Bacillus phage BPS13. (1136731) | |
| Gene Name(s) | No Information Provided. | |
| Protein Name(s) | N-acetylmuramoyl-L-alanine amidase (ECO:0000313 with EMBL:AEZ50187.1) | |
| External Links | ||
| UniProt | J9PU17 | |
| EMBL | JN654439 | |
| RefSeq | YP_006907567.1 | |
| GeneID | 13827971 | |
| KEGG | vg:13827971 | |
| GO | GO:0008745 GO:0009253 | |
| Gene3D | 3.40.80.10 | |
| InterPro | IPR002502 IPR003646 | |
| Pfam | PF01510 PF08460 | |
| SMART | SM00644 SM00287 | |
| SUPFAM | SSF55846 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0008745 |
N-acetylmuramoyl-L-alanine amidase activity |
ECO:0000314 |
F |
Peptidoglycan of B. cereus was incubated with 5 micrograms LysBPS13 for 30 min to determine the amidase activity of LysBPS13 by the amount of free N-acteylmuramic acid liberated. The N-acteylmuramic acid product was first degraded to lactic acid, and then acetaldehyde to be identified colorimetrically with p-hydroxydiphenyl (PHD). The results showed an increase in free N-acteylmuramic acid from cleavage of the bond between N-acetylmuramic acid and L-alanine (Figure 2), demonstrating LysBPS13 has N-acetylmuramyl-L-alanine amidase activity. A glycosidase assay ruled-out LysBPS13 as a glucosaminidase or a muramidase by showing free reducing sugars were not generated by the peptidoglycan after LysBPS13 treatment. Additionally, when 5 micrograms/milliliter of purified, recombinant LysBPS13 was added to B. cereus cells, almost all the B. cereus cells were effectively lysed within 10 min (Figure 1.c), further supporting N-acetylmuramoyl-L-alanine amidase activity of LysBPS13. |
complete | |||||
|
enables |
GO:0008745 |
N-acetylmuramoyl-L-alanine amidase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008745 |
N-acetylmuramoyl-L-alanine amidase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0009253 |
peptidoglycan catabolic process |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 Park, J et al. (2012) Characterization of an endolysin, LysBPS13, from a Bacillus cereus bacteriophage. FEMS Microbiol. Lett. 332 76-83 PubMed GONUTS page
