PMID:7483254

Citation	Haggård-Ljungquist, E, Jacobsen, E, Rishovd, S, Six, EW, Nilssen, O, Sunshine, MG, Lindqvist, BH, Kim, KJ, Barreiro, V and Koonin, EV (1995) Bacteriophage P2: genes involved in baseplate assembly. Virology 213:109-21
Abstract	The sequences of two previously defined tail genes, V and J, of the temperate bacteriophage P2, and those of two new essential tail genes, W and I, were determined. Their order is the late gene promoter, VWJI, followed by the tail fiber genes H and G, and a transcription terminator. The V gene product is the small spike at the tip of the tail, and the J gene product lies at the edge of the baseplate. The W gene product may be homologous to the product of gene 25 of T4 phage, which is part of the T4 baseplate. A temperature-sensitive mutation in gene V affects satellite phage P4 production more than it affects the production of P2 helper phage. P4 mutations that partially compensate for this defect of gene V lie in the P4 capsid size determination gene, sid.
Links	PubMed
Keywords	Amino Acid Sequence; Animals; Bacteriophage P2/genetics; Bacteriophage P2/physiology; Bacteriophage P2/ultrastructure; Base Sequence; Capsid/genetics; Capsid/ultrastructure; Cloning, Molecular; DNA Primers/chemistry; DNA, Viral/analysis; Electrophoresis, Polyacrylamide Gel; Gene Expression Regulation, Viral; Genes, Viral/genetics; Genome, Viral; Molecular Sequence Data; Mutation; Rabbits; Recombinant Proteins; Transcription, Genetic; Viral Proteins/genetics; Viral Proteins/metabolism; Viral Structural Proteins/genetics; Virus Assembly/physiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
9CAUD:A0A024B1F7	Contributes to	GO:0098005: viral head-tail joining	ECO:0000250:	InterPro:IPR006949	P		Baseplate j protein is a component of the baseplate or the proximal part of the tail fiber.	complete CACAO 11516
BPP2:BPJ		GO:0098025: virus tail, baseplate	ECO:0000314:		C		Researchers generated rabbit-based polyclonal antibodies against purified J proteins along with secondary antibodies of biotinylated anti-rabbit IgG conjugated to horseradish peroxidase (Amersham) with immunogold labeling. The results were observed using electron microscopy. Figure 7 shows the presence of gold-conjugated antibodies at the side of the phage baseplate, which causes phage tails to clump together in 58 of the 78 total phage particles observed. The remaining samples were visualized with conjugated gold around the baseplate area. This allowed the researches to conclude with confidence that P2 protein J is a component of the baseplate.	complete CACAO 10852
BPP2:BPJ	part_of	GO:0098025: virus tail, baseplate	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete

References

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