User:Kelizabeth

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	BPP2:BPJ	2015-04-01 10:43:55 CDT	GO:0098025 virus tail, baseplate (C)	PMID:7483254	ECO:0000314 direct assay evidence used in manual assertion	Researchers generated rabbit-based polyclonal antibodies against purified J proteins along with secondary antibodies of biotinylated anti-rabbit IgG conjugated to horseradish peroxidase (Amersham) with immunogold labeling. The results were observed using electron microscopy. Figure 7 shows the presence of gold-conjugated antibodies at the side of the phage baseplate, which causes phage tails to clump together in 58 of the 78 total phage particles observed. The remaining samples were visualized with conjugated gold around the baseplate area. This allowed the researches to conclude with confidence that P2 protein J is a component of the baseplate.	challenge
acceptable	BPT4:TYSY	2015-04-05 16:05:16 CDT	GO:0004799 thymidylate synthase activity (F)	PMID:894793	ECO:0000315 mutant phenotype evidence used in manual assertion	Experiments were performed on wild type T4 td and T4D td6 (a variant containing a missense mutation in the relevant region). A concentration of ~10^13/ml phage ghost particles was collected and thymidylate synthetase activity measured using a radioactive assay of [3H dUMP] for two hours. Table 1 demonstrates thymidylate synthase activity is significantly lower in the td6 mutant (< 0.01 mol/min) than the wild type (0.16 mol/min), indicating that the td gene in T4 phage is involved with thymidylate synthase activity.	challenge
acceptable	9CAUD:S5YCV5	2015-04-06 10:23:16 CDT	GO:0004799 thymidylate synthase activity (F)	GO_REF:0000100	ECO:0000247 sequence alignment evidence used in manual assertion	Targeted BlastP of the Enterobacteriophage T4 td gene (T4p237) against myoviridae shows strong homology with conservation of thymidylate synthase function among hits. Bacillus phage troll gene TROLL_50 (ThyX) displays 99% query cover (Identity 38%) with an e-value of 8e-60, which is within threshold limits. Therefore, it reasonable to assume that the ThyX gene product has a similar thymidylate synthase function to the annotated Enterobacteria phage T4 cousin.	challenge

acceptable:2
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Kelizabeth

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
unacceptable	Gjulie, TeamGreen	9CAUD:S5Y020	GO:0004170 - dUTP diphosphatase activity (F)	GO_REF:0000100	ECO:0000247 sequence alignment evidence used in manual assertion	challenge	C: 1
unacceptable	Gjulie, TeamGreen	9CAUD:S5Y020	GO:0046080 - dUTP metabolic process (P)	PMID:381286	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 2
unacceptable	Csean, TeamPurple	9CAUD:S5Y0M4	GO:0098003 - viral tail assembly (P)	other:GO_REF:0000100	ECO:0000317 genomic context evidence used in manual assertion	challenge	C: 1

0 annotations fixed by Kelizabeth

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