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User:Gjulie

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UMBC Phage Hunters Spring 2015

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptable9CAUD:S5Y0202015-04-06 08:51:31 CDTGO:0046080 regulation of dUTP diphosphatase activity (P)PMID:381286ECO:0000315 mutant phenotype evidence used in manual assertion

The function of this gene in the T5 phage can be concluded from a mutant phenotype. Figure 4 in the referenced paper shows that the dut mutant T5 phage made was not able to induce dUTPase activity. This gives us evidence to conclude that the function of that gene is a dUTPase. IMP can also be assigned to this phage with evidence from an experimental paper that states the function of a dUTPase.

challenge
unacceptable9CAUD:S5Y0202015-04-06 08:51:31 CDTGO:0004170 dUTP diphosphatase activity (F)GO_REF:0000100ECO:0000247 sequence alignment evidence used in manual assertion

BlastP showed that the Troll gene is a homolog to a gene from another bacteriphage, Bacillus phage VB_BCEMBc431v3. The alignment gave an e-value of 1e-122, 93%query cover and 60% identity. Evidence for the function of the Bacillus phage gene is given through experimental evidence done on the phage, T5. The Troll gene also aligned with other dUTP diphosphatase proteins. This is evidence to conclude that the two genes are homologous. The function of this gene is in the reaction that converts dUTP to dUMP.

challenge

acceptable:0
unacceptable:2
requires_changes:0
flagged:0

Annotations challenged by Gjulie

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorWmimi,
TeamBlue
9CAUD:S5Y0L8GO:0098027 - virus tail, sheath (C)GO_REF:0000100ECO:0000247 sequence alignment evidence used in manual assertionchallengeC: 3

0 annotations fixed by Gjulie

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