User:MKoert

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	9CAUD:Q7Y4H8	2018-03-01 12:58:33 CST	GO:0061783 peptidoglycan muralytic activity (F)	PMID:15058185	ECO:0000314 direct assay evidence used in manual assertion	Expression and activity of lytic enzyme was confirmed in Figures 2A/B through SDS Page and an in situ assay. Figure A showed expression of lytic enzyme in ecoli vector plasmid. Figure B showed lytic activity corresponding to the molar mass of a 42kDA protein.	challenge
acceptable	BPHK7:Q9MCS4	2018-03-17 22:14:41 CDT	GO:0098669 superinfection exclusion (P)	PMID:22797755	ECO:0000315 mutant phenotype evidence used in manual assertion	Evidence from figure 1: western blot and plaque assays show HK97 gp15 inhibiting phage infection when compared to a nonsense mutation. It shows this by adding a transformed plasmid into the host cells, one where it expresses gp15 and the other with a nonsense mutation. When expressing gp15 it prevents the growth of HK97 and inhibits the growth of several related phages but there is no similar effect in the nonsense mutation implying that gp15 is the cause of super infection exclusion.	challenge
acceptable	BPHK0:VNUN	2018-04-15 23:19:28 CDT	GO:0032785 negative regulation of DNA-templated transcription, elongation (P)	PMID:24853501	ECO:0000314 direct assay evidence used in manual assertion	Figure 1B shows an assay showing the TEC complex formation time lapsed. The assay shows that in the wild type subjects there was an increased rate of pausing is occurring when compared to the knockout phenotype. Additionally there was shown to be RNAP arrest which can be observed in comparing lanes 3 and 6. This together shows that nun is regulating transcription elongation.	challenge

acceptable:3
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by MKoert

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
updatedbyinstructor	DFrank, Team Green A 2018	9VIRU:A0A088FLK9	GO:0051715 - cytolysis in another organism (P)	PMID:26375388	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1
acceptable	RPatel, Team Green B 2018	I34A1:PA	GO:0004519 - endonuclease activity (F)	PMID:16873236	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1

0 annotations fixed by MKoert

What do the icons mean in the status column?