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9CAUD:Q7Y4H8
Contents
| Species (Taxon ID) | Streptomyces phage mu1/6. (370623) | |
| Gene Name(s) | No Information Provided. | |
| Protein Name(s) | Endolysin (ECO:0000313 with EMBL:AAP84974.1) | |
| External Links | ||
| UniProt | Q7Y4H8 | |
| EMBL | DQ372923 | |
| RefSeq | YP_579222.1 | |
| GeneID | 5076799 | |
| KEGG | vg:5076799 | |
| Proteomes | UP000001778 | |
| Gene3D | 1.10.101.10 | |
| InterPro | IPR002477 | |
| SUPFAM | SSF47090 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0099613 |
protein targeting to cell wall |
ECO:0000314 |
P |
Figure 1 and 3 show that purified proteins from all C-terminal (Cell Wall Binding [CBD]) - Green Flourescent Protein (GFP)fusion constructs, had evident binding activity. In all cases, no binding activity of unfused GFP, used as a negative control, was detected. |
complete | |||||
| GO:0061783 |
peptidoglycan muralytic activity |
ECO:0000314 |
F |
Expression and activity of lytic enzyme was confirmed in Figures 2A/B through SDS Page and an in situ assay. Figure A showed expression of lytic enzyme in ecoli vector plasmid. Figure B showed lytic activity corresponding to the molar mass of a 42kDA protein. |
complete | |||||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Tišáková, L et al. (2014) Bacteriophage endolysin Lyt μ1/6: characterization of the C-terminal binding domain. FEMS Microbiol. Lett. 350 199-208 PubMed GONUTS page
- ↑ Farkasovská, J et al. (2003) Identification and characterization of an endolysin encoded by the Streptomyces aureofaciens phage mu 1/6. Folia Microbiol. (Praha) 48 737-44 PubMed GONUTS page
