GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:15058185
| Citation |
Farkasovská, J, Godány, A and Vlcek, C (2003) Identification and characterization of an endolysin encoded by the Streptomyces aureofaciens phage mu 1/6. Folia Microbiol. (Praha) 48:737-44 |
|---|---|
| Abstract |
An open reading frame homologous to the genes encoding several cell-wall hydrolyzing enzymes was identified on the genome of actinophage mu 1/6. This open reading frame encoding the putative endolysin was amplified by polymerase chain reaction and cloned into the expression vector pET-21a. This gene consisted of 1182 bp encoding a 393 amino acid polypeptide with a molar mass of 42.1 kDa. The gene product was overexpressed in Escherichia coli, and then the lytic enzyme was purified by a two-step chromatographic procedure. When applied exogenously, the endolysin of phage mu 1/6 was active against all tested Streptomyces strains but did not affect other bacteria. The amino acid sequence showed a high homology with a putative amidase of the Streptomyces phase phi C31. Downstream of the endolysin gene, an open reading frame encoding an 88 amino acid protein was identified. Structural analysis of its sequence revealed features characteristics for holin. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacteriophage mu/enzymology; Bacteriophage mu/genetics; Cell Wall/metabolism; Cloning, Molecular; Endopeptidases/genetics; Endopeptidases/isolation & purification; Endopeptidases/metabolism; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Viral; Hydrogen-Ion Concentration; Hydrolysis; Molecular Sequence Data; Streptomyces aureofaciens/virology |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0061783: peptidoglycan muralytic activity |
ECO:0000314: |
F |
Expression and activity of lytic enzyme was confirmed in Figures 2A/B through SDS Page and an in situ assay. Figure A showed expression of lytic enzyme in ecoli vector plasmid. Figure B showed lytic activity corresponding to the molar mass of a 42kDA protein. |
complete | ||||
Notes
See also
References
See Help:References for how to manage references in GONUTS.
