GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
BPHK0:VNUN
Contents
| Species (Taxon ID) | Enterobacteria phage HK022 (Bacteriophage HK022). (10742) | |
| Gene Name(s) | nun | |
| Protein Name(s) | Transcription termination factor nun | |
| External Links | ||
| UniProt | P18683 | |
| EMBL | X16093 | |
| PIR | S06539 | |
| PDB | 1E4Z 1HJI | |
| PDBsum | 1E4Z 1HJI | |
| ProteinModelPortal | P18683 | |
| SMR | P18683 | |
| EvolutionaryTrace | P18683 | |
| GO | GO:0006353 GO:0006355 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0006353 |
DNA-templated transcription, termination |
ECO:0000314 |
P |
Figure 2 - In an in vitro system with RNA polymerase and the nun protein, increasing concentration of nun correlated with increasing amounts of relatively shorter RNA transcripts. (Data not shown) - Adding the nun protein after completion of transcription did not shorten RNA transcripts. Thus, its unlikely nun acts by cleaving or processing RNA transcripts. Figure 3A - Without nun, RNA polymerase transcription would only pause at certain lengths of RNA, before resuming activity again. However in presence of nun, transcription would completely be completely blocked without resuming again once these similar length RNA transcripts were reached. Both with and without nun, similar length RNA transcripts would appear at similar times. Figure 3B - Next, the authors separated the the RNA transcript products onto a gel. They found that the sites that nun acted on and the sites where RNA polymerase paused were either identical or extremely close in proximity. All these results taken into consideration suggest nun arrests transcription by directly acting at RNA polymerase pause sites. |
complete | |||||
| GO:0032785 |
negative regulation of DNA-templated transcription, elongation |
ECO:0000314 |
P |
Figure 1B shows an assay showing the TEC complex formation time lapsed. The assay shows that in the wild type subjects there was an increased rate of pausing is occurring when compared to the knockout phenotype. Additionally there was shown to be RNAP arrest which can be observed in comparing lanes 3 and 6. This together shows that nun is regulating transcription elongation. |
complete | |||||
|
involved_in |
GO:0006353 |
DNA-templated transcription, termination |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Hung, SC & Gottesman, ME (1995) Phage HK022 Nun protein arrests transcription on phage lambda DNA in vitro and competes with the phage lambda N antitermination protein. J. Mol. Biol. 247 428-42 PubMed GONUTS page
- ↑ Vitiello, CL et al. (2014) Coliphage HK022 Nun protein inhibits RNA polymerase translocation. Proc. Natl. Acad. Sci. U.S.A. 111 E2368-75 PubMed GONUTS page
