Cacao

(Data not shown) - Adding the nun protein after completion of transcription did not shorten RNA transcripts. Thus, its unlikely nun acts by cleaving or processing RNA transcripts. Figure 3A - Without nun, RNA polymerase transcription would only pause at certain lengths of RNA, before resuming activity again. However in presence of nun, transcription would completely be completely blocked without resuming again once these similar length RNA transcripts were reached. Both with and without nun, similar length RNA transcripts would appear at similar times. Figure 3B - Next, the authors separated the the RNA transcript products onto a gel. They found that the sites that nun acted on and the sites where RNA polymerase paused were either identical or extremely close in proximity. All these results taken into consideration suggest nun arrests transcription by directly acting at RNA polymerase pause sites.||complete
CACAO 12477