User:Khana25

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	BPA51:A8ASU2	2016-04-22 10:38:45 CDT	GO:0098024 virus tail, fiber (C)	PMID:24673724	ECO:0000314 direct assay evidence used in manual assertion	Figure 7. Panel O, shows the gp108 tail fiber in electron microscopy, at the appropriate location for the function of a tail fiber. Specifically, the immunogold particles binding near the baseplate, which is where you would expect to see the tail fiber.	challenge
updatedbyinstructor	9CAUD:S4U6W5	2016-04-25 10:33:56 CDT	GO:0019062 virion attachment to host cell (P)	GO_REF:0000100	ECO:0000247 sequence alignment evidence used in manual assertion	Blastp results for transfer annotation yield an E-value of 0.0, Identity similarity of 98.1%, and a Query Coverage of 100%, as evidence for sequence similarity	challenge
updatedbyinstructor	9CAUD:A0A059T853	2016-04-25 10:47:19 CDT	GO:0019062 virion attachment to host cell (P)	GO_REF:0000100	ECO:0000247 sequence alignment evidence used in manual assertion	Blastp results for transfer annotation yield an E-value of 0.0, Identity similarity of 98.1%, and a Query Coverage of 100%, as evidence for sequence similarity.	challenge
updatedbyinstructor	9CAUD:A0A059T6U2	2016-04-27 01:54:10 CDT	GO:0019062 virion attachment to host cell (P)	GO_REF:0000100	ECO:0000247 sequence alignment evidence used in manual assertion	Blastp results for transfer annotation yield an E-value of 0.0, Identity similarity of 97.7%, and a Query Coverage of 100%, as evidence for sequence similarity.	challenge
updatedbyinstructor	9CAUD:A0A059T8Q0	2016-04-27 02:00:44 CDT	GO:0019062 virion attachment to host cell (P)	GO_REF:0000100	ECO:0000247 sequence alignment evidence used in manual assertion	Blastp results for transfer annotation yield an E-value of 0.0, Identity similarity of 97.7%, and a Query Coverage of 100%, as evidence for sequence similarity.	challenge
updatedbyinstructor	9CAUD:A0A060AKZ1	2016-04-27 02:14:14 CDT	GO:0019062 virion attachment to host cell (P)	GO_REF:0000100	ECO:0000247 sequence alignment evidence used in manual assertion	Blastp results for transfer annotation yield an E-value of 0.0, Identity similarity of 97.0%, and a Query Coverage of 100%, as evidence for sequence similarity.	challenge
updatedbyinstructor	9CAUD:A0A068CCS0	2016-04-27 02:29:28 CDT	GO:0019062 virion attachment to host cell (P)	GO_REF:0000100	ECO:0000247 sequence alignment evidence used in manual assertion	Blastp results for transfer annotation yield an E-value of 0.0, Identity similarity of 97.2%, and a Query Coverage of 100%, as evidence for sequence similarity.	challenge
updatedbyinstructor	BPA51:A8ASU2	2016-05-02 09:26:44 CDT	GO:0019062 virion attachment to host cell (P)	PMID:24673724	ECO:0000314 direct assay evidence used in manual assertion	Figure 2 shows how the antibody of gp108 tail fiber protein inhibits adsorption of bacteriophage A511 to the WSLC 3009 host cell. The bars on the graphs above the label WSLC3009 + gp108 show a significant decrease in efficiency in plating and adorption. This indicates the antibody gp108's ability to block the binding of the A511 to the host cell.	challenge
unacceptable	9CAUD:Q8LTE6	2016-05-16 11:56:38 CDT	GO:0009253 peptidoglycan catabolic process (P)	PMID:18662316	ECO:0000247 sequence alignment evidence used in manual assertion	Figure 1a in the literature shows a schematic representation of PlyG and PlyL, and the umbers of amino acid residues involved in catalysis are respectively, 233 and 234, indicating a high sequence similarity and low E-value, allowing assumption of function similarity to the endolysin PlyL. In addition, figure 2a, shows a sequence alignment of PlyG and PlyL and PlyG has a high degree of sequence similarity to PlyL. There are only 10 amino acid residue differences within the N-terminal regions of PlyG and PlyL, while 30 amino acid residues are different within their C-terminal regions. Therefore, it seems likely that the N-terminal region of PlyG also has similar characteristics.	challenge
updatedbyinstructor	9CAUD:Q8LTE6	2016-05-16 17:17:28 CDT	GO:0051672 catabolism by organism of cell wall peptidoglycan in other organism (P)	PMID:18662316	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2d. in the literature compares Lytic activities of wild-type PlyG and its C-terminal deletion mutant. Catalytic activity was examined by colony-forming assay, and the control, PlyGB, was set to 100%. As shown in the figure, deletion of the C-terminal region completely abolished the lytic ability of PlyG, with the colony forming units of the C-terminal deletion mutant at a very high percentage, versus the normal wild type PlyG, which yielded a very low colony forming unit percentage. This indicates that the cell-wall-binding domain of PlyG is essential for its catalytic activity, and that addition of PlyG restricts colonies of bacteria from forming, characterizing it as an endolysin with peptidoglycan catabolic process and cleavage.	challenge

acceptable:0
unacceptable:1
requires_changes:0
flagged:0

Annotations challenged by Khana25

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
unacceptable	E63, Team Blue A	9CAUD:A0A0K2D000	GO:0004146 - dihydrofolate reductase activity (F)	PMID:10731419	ECO:0000250 sequence similarity evidence used in manual assertion	challenge	C: 5
unacceptable	Mnguy1, Team Blue B	BPT7:DPOL	GO:0004527 - exonuclease activity (F)	2703498:2703498	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 5
updatedbyinstructor	Barlowrm, Team TRUMP2016	BACSU:TYSY1	GO:0004799 - thymidylate synthase activity (F)	PMID:9778348	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 4
unacceptable	Heatheca, Team ContaminatorZ	9CAUD:A0A024B0X2	GO:0098020 - obsolete virus tail, minor subunit (C)	PMID:22923442	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 5
updatedbyinstructor	Dalton.Fellows, Team Tiger2	BPMD2:VG65	GO:0008310 - single-stranded DNA 3'-5' DNA exonuclease activity (F)	PMID:19028888	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1

0 annotations fixed by Khana25

What do the icons mean in the status column?