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User:Barlowrm

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Phage Hunters Spring 2016

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorBACSU:TYSY12016-04-05 07:33:24 CDTGO:0004799 thymidylate synthase activity (F)PMID:9778348ECO:0000314 direct assay evidence used in manual assertion

"Activity assays indicate that bsTS-A has a significantly higher specific activity (SA ≈ 22 units/mg) than either E. coli TS (SA ∼13.5 units/mg) or L. casei TS (SA ≈ 6 units/mg) when measured using the identical materials and instruments." Data not shown.

challenge
unacceptableHUMAN:HPRT2016-04-11 11:22:55 CDTGO:0000100 S-methylmethionine transmembrane transporter activity (F)PMID:17662107ECO:0000250 sequence similarity evidence used in manual assertion

The main protein ribose-phosphate pyrophosphokinase from the Hakuna Genome, shares a homology with guanine phosphoribosyltransferase with an e score of 4e-10 according to HHPRED. The function and structure of this protein is to design compounds that would be effective inhibitors. "Almost all of the inhibitors available are analogues of the substrate or transition-state. The main drawback of these compounds is that there is poor differential inhibition among various HGPRTs. The reason maybe due to high structure similarity of HGPRTs from different species (especially the active site) even though there is only moderate sequence homology. The key residues in the active site are highly similar among HGPRTs (Table 1)."

challenge
unacceptableHUMAN:PNCB2016-04-11 03:20:12 CDTGO:0004516 nicotinate phosphoribosyltransferase activity (F)PMID:18321072ECO:0000314 direct assay evidence used in manual assertion

According to HHPRED the protein nicotinate phosphoribosyltransferase shares a homology with the protein Quinolinate Phosphoribosyl Transferase, with an e value of 1.2E-12. Figure 3 shows the structural homology between Dimer of scQAPRTase complexed with phthalate and PRPP. (B) Hexamer of aposcQAPRTase. The function of nicotinate phosphoribosyltransferase is to catalyze the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN).

challenge
unacceptable9CAUD:A0A024B0Y12016-04-23 16:14:39 CDTGO:0000100 S-methylmethionine transmembrane transporter activity (F)PMID:24004689ECO:0000250 sequence similarity evidence used in manual assertion

Figure 3 shows a multiple sequence alignments of representative sequences in the DUF4424 and YARHG families.

challenge
unacceptableARATH:PPA262016-04-23 17:58:34 CDTGO:1904097 acid phosphatase complex (C)PMID:25075326ECO:0000314 direct assay evidence used in manual assertion

The homohexameric organization of PPD1, as revealed by a 1.65 Å resolution crystal structure and confirmed by solution X-ray scattering, is unique among plant PAPs, for which only homodimers have previously been reported. A phosphate anion is bound in a bidentate fashion at the active site, bridging the Fe and Mn atoms in a binding mode similar to that previously reported for sweet potato PAP, which suggests that common features occur in their catalytic mechanisms.

challenge
unacceptableHUMAN:LKHA42016-05-04 15:45:10 CDTGO:0000175 3'-5'-exoribonuclease activity (F)PMID:24558481ECO:0000250 sequence similarity evidence used in manual assertion

HHPRED shows an e value of 1.4E-13. Figure two from the article shows sequence similarity between the two proteins. Along with a gel electrophoresis comparing the bands between of dna between the two proteins

challenge
unacceptableLUPLU:Q8VX112016-05-16 08:48:00 CDTGO:1904097 acid phosphatase complex (C)PMID:25075326ECO:0000314 direct assay evidence used in manual assertion

The homohexameric organization of PPD1, as revealed by a 1.65 Å resolution crystal structure and confirmed by solution X-ray scattering, is unique among plant PAPs, for which only homodimers have previously been reported. A phosphate anion is bound in a bidentate fashion at the active site, bridging the Fe and Mn atoms in a binding mode similar to that previously reported for sweet potato PAP, which suggests that common features occur in their catalytic mechanisms.

challenge
unacceptableCALS4:Q8R7L02016-05-16 09:00:05 CDTGO:0000100 S-methylmethionine transmembrane transporter activity (F)PMID:17662107ECO:0000250 sequence similarity evidence used in manual assertion

The function and structure of this protein is to design compounds that would be effective inhibitors. "Almost all of the inhibitors available are analogues of the substrate or transition-state. The main drawback of these compounds is that there is poor differential inhibition among various HGPRTs. The reason maybe due to high structure similarity of HGPRTs from different species (especially the active site) even though there is only moderate sequence homology. The key residues in the active site are highly similar among HGPRTs (Table 1)."

challenge
unacceptableTRYB2:Q38AY42016-05-16 09:04:52 CDTGO:0005634 nucleus (C)PMID:24558481ECO:0000250 sequence similarity evidence used in manual assertion

Figure two from the article shows sequence similarity between the two proteins. Along with a gel electrophoresis comparing the bands between of dna between the two proteins

challenge

acceptable:0
unacceptable:8
requires_changes:0
flagged:0

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