Phage Hunters Spring 2016
My Annotations
Status | Page | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
---|
updatedbyinstructor | BACSU:TYSY1 | 2016-04-05 07:33:24 CDT | GO:0004799 thymidylate synthase activity (F) | PMID:9778348 | ECO:0000314 direct assay evidence used in manual assertion | "Activity assays indicate that bsTS-A has a significantly higher specific activity (SA ≈ 22 units/mg) than either E. coli TS (SA ∼13.5 units/mg) or L. casei TS (SA ≈ 6 units/mg) when measured using the identical materials and instruments." Data not shown.
| challenge |
unacceptable | HUMAN:HPRT | 2016-04-11 11:22:55 CDT | GO:0000100 S-methylmethionine transmembrane transporter activity (F) | PMID:17662107 | ECO:0000250 sequence similarity evidence used in manual assertion | The main protein ribose-phosphate pyrophosphokinase from the Hakuna Genome, shares a homology with guanine phosphoribosyltransferase with an e score of 4e-10 according to HHPRED. The function and structure of this protein is to design compounds that would be effective inhibitors. "Almost all of the inhibitors available are analogues of the substrate or transition-state. The main drawback of these compounds is that there is poor differential inhibition among various HGPRTs. The reason maybe due to high structure similarity of HGPRTs from different species (especially the active site) even though there is only moderate sequence homology. The key residues in the active site are highly similar among HGPRTs (Table 1)."
| challenge |
unacceptable | HUMAN:PNCB | 2016-04-11 03:20:12 CDT | GO:0004516 nicotinate phosphoribosyltransferase activity (F) | PMID:18321072 | ECO:0000314 direct assay evidence used in manual assertion | According to HHPRED the protein nicotinate phosphoribosyltransferase shares a homology with the protein Quinolinate Phosphoribosyl Transferase, with an e value of 1.2E-12. Figure 3 shows the structural homology between Dimer of scQAPRTase complexed with phthalate and PRPP. (B) Hexamer of aposcQAPRTase. The function of nicotinate phosphoribosyltransferase is to catalyze the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN).
| challenge |
unacceptable | 9CAUD:A0A024B0Y1 | 2016-04-23 16:14:39 CDT | GO:0000100 S-methylmethionine transmembrane transporter activity (F) | PMID:24004689 | ECO:0000250 sequence similarity evidence used in manual assertion | Figure 3 shows a multiple sequence alignments of representative sequences in the DUF4424 and YARHG families.
| challenge |
unacceptable | ARATH:PPA26 | 2016-04-23 17:58:34 CDT | GO:1904097 acid phosphatase complex (C) | PMID:25075326 | ECO:0000314 direct assay evidence used in manual assertion | The homohexameric organization of PPD1, as revealed by a 1.65 Å resolution crystal structure and confirmed by solution X-ray scattering, is unique among plant PAPs, for which only homodimers have previously been reported. A phosphate anion is bound in a bidentate fashion at the active site, bridging the Fe and Mn atoms in a binding mode similar to that previously reported for sweet potato PAP, which suggests that common features occur in their catalytic mechanisms.
| challenge |
unacceptable | HUMAN:LKHA4 | 2016-05-04 15:45:10 CDT | GO:0000175 3'-5'-exoribonuclease activity (F) | PMID:24558481 | ECO:0000250 sequence similarity evidence used in manual assertion | HHPRED shows an e value of 1.4E-13. Figure two from the article shows sequence similarity between the two proteins. Along with a gel electrophoresis comparing the bands between of dna between the two proteins
| challenge |
unacceptable | LUPLU:Q8VX11 | 2016-05-16 08:48:00 CDT | GO:1904097 acid phosphatase complex (C) | PMID:25075326 | ECO:0000314 direct assay evidence used in manual assertion | The homohexameric organization of PPD1, as revealed by a 1.65 Å resolution crystal structure and confirmed by solution X-ray scattering, is unique among plant PAPs, for which only homodimers have previously been reported. A phosphate anion is bound in a bidentate fashion at the active site, bridging the Fe and Mn atoms in a binding mode similar to that previously reported for sweet potato PAP, which suggests that common features occur in their catalytic mechanisms.
| challenge |
unacceptable | CALS4:Q8R7L0 | 2016-05-16 09:00:05 CDT | GO:0000100 S-methylmethionine transmembrane transporter activity (F) | PMID:17662107 | ECO:0000250 sequence similarity evidence used in manual assertion | The function and structure of this protein is to design compounds that would be effective inhibitors. "Almost all of the inhibitors available are analogues of the substrate or transition-state. The main drawback of these compounds is that there is poor differential inhibition among various HGPRTs. The reason maybe due to high structure similarity of HGPRTs from different species (especially the active site) even though there is only moderate sequence homology. The key residues in the active site are highly similar among HGPRTs (Table 1)."
| challenge |
unacceptable | TRYB2:Q38AY4 | 2016-05-16 09:04:52 CDT | GO:0005634 nucleus (C) | PMID:24558481 | ECO:0000250 sequence similarity evidence used in manual assertion | Figure two from the article shows sequence similarity between the two proteins. Along with a gel electrophoresis comparing the bands between of dna between the two proteins
| challenge |
acceptable:0
unacceptable:8
requires_changes:0
flagged:0
What do the icons mean in the status column?