User:Ka043496

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	ECOLX:G9IBF5	2012-04-01 16:10:09 CDT	GO:0007050 cell cycle arrest (P)	PMID:18005237	ECO:0000314 direct assay evidence used in manual assertion	The results of FACS (fluorescence activated cell sorting) is shown in figure 4. HeLa cells were treated with SubAB and by hour 20 it was evident that there was a definite increase in the number of cells in G0-G1 phase compared to time 0 as well as a decrease in the number of cells in the other stages. Figure 4 shows that SubAB causes cells to arrest in G1.	challenge
requireschanges	ECOLX:G9IBF5	2012-04-01 16:30:33 CDT	GO:0007050 cell cycle arrest (P)	PMID:18005237	ECO:0000314 direct assay evidence used in manual assertion	Figure 5 shows a western blot looking at the effect SubAB has on the levels of cyclin D1, cyclin D3, Cdk4, and Cdk6. This experiment was done to examine the underlying cause of cell cycle arrest. A significant downregulation of cyclin D1 occurred between 30 and 60 minutes after exposure to SubAB. Cyclin D is a G1 cyclin and the downregulation of cyclin D1 led to arrest in G1 of the cell cycle.	challenge
requireschanges	ECOLX:G9IBF5	2012-04-01 17:30:57 CDT	GO:0006508 proteolysis (P)	PMID:17024087	ECO:0000314 direct assay evidence used in manual assertion	Figure 1B is a western blot which shows the cleavage of BiP by SubAB over a time span of 60 minutes. The antibody used was polyclonal goat anti-BiP. At 20 minutes the band at 28-kDa (part of the cleaved protein) became evident and by 30 minutes it was evident that the band at 72-kDa (the intact protein) had become diminished. Only one cleavage fragment was visible because when the antibody was made, it was made against a peptide from the C-terminus of BiP so only the intact and C terminal portion of the protein would bind to the antibody and produce a band.	challenge

acceptable:0
unacceptable:0
requires_changes:3
flagged:0

Annotations challenged by Ka043496

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
unacceptable	An553775, Team Jacques Monod	FRANO:Q7X3J5	GO:0005625 - obsolete soluble fraction (C)	PMID:17233889	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1
requireschanges	El001607, Team Kary Mullis	AZOVI:Q9KIB6	GO:0045893 - positive regulation of DNA-templated transcription (P)	PMID:10940024	ECO:0000316 genetic interaction evidence used in manual assertion	challenge	C: 2

0 annotations fixed by Ka043496

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	HELPX:C7F7V2	2012-02-22 18:52:02 CST	GO:0005773 vacuole (C)	PMID:16436379	ECO:0000314 direct assay evidence used in manual assertion	Using immunocytochemistry and confocal analysis, figure 2 shows that VacA is localized to vacuoles, but not to mitochondria.	challenge
requireschanges	HELPX:C7F7V2	2012-02-21 22:28:59 CST	GO:0009405 pathogenesis (P)	PMID:16436379	ECO:0000314 direct assay evidence used in manual assertion	Figure 4 (A-F) shows flow cytometric analysis using antibodies specific for active Bax to compare cells that were treated with VacA to cells that were not. Bax was activated when exposed to VacA.	challenge

acceptable:0
unacceptable:0
requires_changes:2
flagged:0

Annotations challenged by Ka043496

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Ka043496