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PMID:10940024
| Citation |
Núñez, C, Moreno, S, Cárdenas, L, Soberón-Chávez, G and Espín, G (2000) Inactivation of the ampDE operon increases transcription of algD and affects morphology and encystment of Azotobacter vinelandii. J. Bacteriol. 182:4829-35 |
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| Abstract |
Transcription of algD, encoding GDP-mannose dehydrogenase, the key enzyme in the alginate biosynthetic pathway, is highly regulated in Azotobacter vinelandii. We describe here the characterization of a Tn5 insertion mutant (AC28) which shows a higher level of expression of an algD::lacZ fusion. AC28 cells were morphologically abnormal and unable to encyst. The cloning and nucleotide sequencing of the Tn5-disrupted locus in AC28 revealed an operon homologous to the Escherichia coli ampDE operon. Tn5 was located within the ampD gene, encoding a cytosolic N-acetyl-anhydromuramyl-L-alanine amidase that participates in the intracellular recycling of peptidoglycan fragments. The ampE gene encodes a transmembrane protein, but the function of the protein is not known. We constructed strains carrying ampD or ampE mutations and one with an ampDE deletion. The strain with a deletion of the ampDE operon showed a phenotype similar to that of mutant AC28. The present work demonstrates that both alginate production and bacterial encystment are greatly influenced by the bacterial ability to recycle its cell wall. |
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| Keywords |
Alginates/metabolism; Azotobacter vinelandii/enzymology; Azotobacter vinelandii/genetics; Azotobacter vinelandii/isolation & purification; Azotobacter vinelandii/physiology; Bacterial Proteins/genetics; Base Sequence; Carbohydrate Dehydrogenases/genetics; Cloning, Molecular; DNA, Bacterial; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Genes, Bacterial; Glucuronic Acid; Hexuronic Acids; Membrane Proteins/genetics; Molecular Sequence Data; Mutagenesis; N-Acetylmuramoyl-L-alanine Amidase/genetics; Operon; Promoter Regions, Genetic; Sequence Analysis, DNA; Transcription, Genetic; Transposases; beta-Galactosidase/genetics; beta-Galactosidase/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0045893: positive regulation of transcription, DNA-dependent |
ECO:0000316: |
UniProtKB:Q9KIB5
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P |
Figure 2 |
complete | |||
See also
References
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