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ECOLX:G9IBF5
Contents
| Species (Taxon ID) | Escherichia coli. (562) | |
| Gene Name(s) | subAB2 (ECO:0000313 with EMBL:AEU11063.1) | |
| Protein Name(s) | Subtilase cytotoxin (ECO:0000313 with EMBL:AEU11063.1) | |
| External Links | ||
| UniProt | G9IBF5 | |
| EMBL | JN638553 | |
| ProteinModelPortal | G9IBF5 | |
| GO | GO:0004252 | |
| Gene3D | 3.40.50.200 | |
| InterPro | IPR000209 | |
| Pfam | PF00082 | |
| SUPFAM | SSF52743 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0007050 |
cell cycle arrest |
ECO:0000314 |
P |
The results of FACS (fluorescence activated cell sorting) is shown in figure 4. HeLa cells were treated with SubAB and by hour 20 it was evident that there was a definite increase in the number of cells in G0-G1 phase compared to time 0 as well as a decrease in the number of cells in the other stages. Figure 4 shows that SubAB causes cells to arrest in G1. |
complete | |||||
| GO:0007050 |
cell cycle arrest |
ECO:0000314 |
P |
Figure 5 shows a western blot looking at the effect SubAB has on the levels of cyclin D1, cyclin D3, Cdk4, and Cdk6. This experiment was done to examine the underlying cause of cell cycle arrest. A significant downregulation of cyclin D1 occurred between 30 and 60 minutes after exposure to SubAB. Cyclin D is a G1 cyclin and the downregulation of cyclin D1 led to arrest in G1 of the cell cycle. |
complete | |||||
| GO:0006508 |
proteolysis |
ECO:0000314 |
P |
Figure 1B is a western blot which shows the cleavage of BiP by SubAB over a time span of 60 minutes. The antibody used was polyclonal goat anti-BiP. At 20 minutes the band at 28-kDa (part of the cleaved protein) became evident and by 30 minutes it was evident that the band at 72-kDa (the intact protein) had become diminished. Only one cleavage fragment was visible because when the antibody was made, it was made against a peptide from the C-terminus of BiP so only the intact and C terminal portion of the protein would bind to the antibody and produce a band. |
complete | |||||
|
enables |
GO:0004252 |
serine-type endopeptidase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006508 |
proteolysis |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 Morinaga, N et al. (2008) Subtilase cytotoxin, produced by Shiga-toxigenic Escherichia coli, transiently inhibits protein synthesis of Vero cells via degradation of BiP and induces cell cycle arrest at G1 by downregulation of cyclin D1. Cell. Microbiol. 10 921-9 PubMed GONUTS page
- ↑ Paton, AW et al. (2006) AB5 subtilase cytotoxin inactivates the endoplasmic reticulum chaperone BiP. Nature 443 548-52 PubMed GONUTS page
