User:Jluo23

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	PSEAI:P72137	2013-09-15 16:54:53 CDT	GO:0030171 voltage-gated proton channel activity (F)	PMID:24023388	ECO:0000314 direct assay evidence used in manual assertion	I- flux was shown to significantly increase (~200% at pH 6.5 compared to pH 7.5) when proteoliposome external pH was decreased in Fig 2 which indicates Wzx's the proton-dependent transporter activity.	challenge
updatedbyinstructor	PHAVU:G1FTR5	2013-09-15 17:24:54 CDT	GO:0004190 aspartic-type endopeptidase activity (F)	PMID:21985276	ECO:0000315 mutant phenotype evidence used in manual assertion	Table 2	challenge
updatedbyinstructor	ALIFS:Q6U738	2013-09-15 17:58:45 CDT	GO:0035556 intracellular signal transduction (P)	PMID:14645281	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig. 3 demonstrate that one of the Vibrio fischeri GacA global regulator functions is in luminescence. Mutants are defective in luminescence by measuring LU/OD600.	challenge
requireschanges	HELPX:P94835	2013-09-29 16:00:53 CDT	GO:0072321 chaperone-mediated protein transport (P)	PMID:24072713	ECO:0000314 direct assay evidence used in manual assertion	Figure 1 shows CagF interaction with CagA using calorimetric analysis; CagF chaperone mediates CagA translocation into gastric epithelial cells.	challenge
updatedbyinstructor	HELPX:P94835	2013-09-29 16:09:18 CDT	GO:0042000 translocation of peptides or proteins into host (P)	PMID:17768234	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1	challenge
updatedbyinstructor	HELPX:Q9JP55	2013-09-29 16:18:09 CDT	GO:0070062 extracellular vesicular exosome (C)	PMID:20659286	ECO:0000314 direct assay evidence used in manual assertion	Figure 8 shows immunogold analysis of CagA, demonstrating localization to bacterial vesicle surface.	challenge
unacceptable	ACEPA:C7K2B1	2013-10-13 14:48:49 CDT	GO:0003899 DNA-directed RNA polymerase activity (Fig. 1 shows a decreased production of chaperones after heat shock in rpoH mutant strain compared to parental strain.)	PMID:21239225	IMP: Inferred from Mutant Phenotype		challenge
unacceptable	ACEPA:C7K2B1	2013-10-13 14:53:44 CDT	GO:0016987 sigma factor activity (Fig. 3 demonstrates that rpoH mutants have significantly decreased survival when presented with heat, ethanol, and acid stress.)	PMID:21239225	IMP: Inferred from Mutant Phenotype		challenge
unacceptable	DROME:DYRK2	2013-10-27 15:15:13 CDT	GO:0042048 olfactory behavior (P)	PMID:24146926	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2 shows a t-maze assay using 1% benzaldehyde. Aversion was calculated with a response index and both mutant male and female DmDyrk2−/− mutants showed significantly less aversion to benzaldehyde.	challenge
acceptable	SALTM:O84946	2013-10-27 16:00:48 CDT	GO:0072321 chaperone-mediated protein transport (P)	PMID:24090070	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3 western blot of WT SscA and mutant SscA demonstrate that secretion of SseC is mediated by SscA.	challenge

acceptable:2
unacceptable:3
requires_changes:1
flagged:0

Annotations challenged by Jluo23

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

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