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Category:Team JAAM

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableEMENI:CHSCAskarlupka, Team JAAM2013-11-10 15:41:25 CSTGO:0000918 barrier septum site selection (P)PMID:15947204ECO:0000316 genetic interaction evidence used in manual assertion

Figure 1 and Figure 2. The double deletion mutant of chsA and chsC showed septum formation in abnormal locations. The septa were formed closer together in the double deletion mutant compared to the wild type and the single deletion mutants.

challenge
acceptableEMENI:CHSCAskarlupka, Team JAAM2013-11-10 15:40:30 CSTGO:0048315 conidium formation (P)PMID:15947204ECO:0000316 genetic interaction evidence used in manual assertion

Table 2. Conidiation of the chsA and chsC double mutant was immeasurable.

challenge
acceptableEMENI:CHSAAskarlupka, Team JAAM2013-11-10 15:36:55 CSTGO:0048315 conidium formation (P)PMID:15947204ECO:0000316 genetic interaction evidence used in manual assertion

Table 2. Conidiation of the chsA and chsC double mutant was immeasurable.

challenge
acceptableEMENI:CHSAAskarlupka, Team JAAM2013-11-10 15:11:02 CSTGO:0000918 barrier septum site selection (P)PMID:15947204ECO:0000316 genetic interaction evidence used in manual assertion

Figure 1 and Figure 2. The double deletion mutant of chsA and chsC showed septum formation in abnormal locations. The septa were formed closer together in the double deletion mutant compared to the wild type and the single deletion mutants.

challenge
unacceptableSINMM:F7X1J5Mjdolan2, Team JAAM2013-11-06 01:09:23 CSTGO:0003824 catalytic activity (F)PMID:15806287ECO:0000316 genetic interaction evidence used in manual assertion

NtrC activation of promoter. Fig 3

challenge
requireschangesHALMT:B8ZYW0Mjdolan2, Team JAAM2013-11-04 23:22:38 CSTGO:0003824 catalytic activity (F)PMID:24039236ECO:0000316 genetic interaction evidence used in manual assertion

Figure 5

challenge
acceptableSALTM:O84946Jluo23, Team JAAM2013-10-27 16:00:48 CDTGO:0072321 chaperone-mediated protein transport (P)PMID:24090070ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 western blot of WT SscA and mutant SscA demonstrate that secretion of SseC is mediated by SscA.

challenge
unacceptableDROME:DYRK2Jluo23, Team JAAM2013-10-27 15:15:13 CDTGO:0042048 olfactory behavior (P)PMID:24146926ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 shows a t-maze assay using 1% benzaldehyde. Aversion was calculated with a response index and both mutant male and female DmDyrk2−/− mutants showed significantly less aversion to benzaldehyde.

challenge
updatedbyinstructorEMENI:CHSCAskarlupka, Team JAAM2013-10-13 16:59:24 CDTGO:0001411 hyphal tip (C)PMID:15947204ECO:0000314 direct assay evidence used in manual assertion

Figure 7. Using indirect immunofluorescence the localization of the FLAG-ChsC flagged protein was found to be located at the hyphal tips.

challenge
unacceptableACEPA:C7K2B1Jluo23, Team JAAM2013-10-13 14:53:44 CDTGO:0016987 sigma factor activity (Fig. 3 demonstrates that rpoH mutants have significantly decreased survival when presented with heat, ethanol, and acid stress.)PMID:21239225IMP: Inferred from Mutant Phenotype
challenge
unacceptableACEPA:C7K2B1Jluo23, Team JAAM2013-10-13 14:48:49 CDTGO:0003899 DNA-directed RNA polymerase activity (Fig. 1 shows a decreased production of chaperones after heat shock in rpoH mutant strain compared to parental strain.)PMID:21239225IMP: Inferred from Mutant Phenotype
challenge
unacceptableEMENI:CHSCAskarlupka, Team JAAM2013-10-12 00:11:39 CDTGO:0048315 conidium formation (P)PMID:15947204ECO:0000316 genetic interaction evidence used in manual assertion

Table 2. Conidiation of the chsA and chsC double mutant was immeasurable.

challenge
unacceptableEMENI:CHSAAskarlupka, Team JAAM2013-10-11 18:56:57 CDTGO:0048315 conidium formation (P)PMID:15947204ECO:0000316 genetic interaction evidence used in manual assertion

Table 2. Conidiation of the chsA and chsC double mutant was immeasurable.

challenge
requireschangesEMENI:CHSAAskarlupka, Team JAAM2013-10-11 18:56:57 CDTGO:1902432 conidium formation (P)PMID:15947204ECO:0000316 genetic interaction evidence used in manual assertion

Figure 5. Using indirect immunofluorescence the localization of the HA-ChsA flagged protein was found to be at the septa.

challenge
unacceptableEMENI:CHSCAskarlupka, Team JAAM2013-10-11 15:59:17 CDTGO:0000918 barrier septum site selection (P)PMID:15947204ECO:0000316 genetic interaction evidence used in manual assertion

Figure 1 and Figure 2. The double deletion mutant of chsA and chsC showed septum formation in abnormal locations. The septa were formed closer together in the double deletion mutant compared to the wild type and the single deletion mutants.

challenge
unacceptableEMENI:CHSAAskarlupka, Team JAAM2013-10-11 15:57:05 CDTGO:0000918 barrier septum site selection (P)PMID:15947204ECO:0000316 genetic interaction evidence used in manual assertion

Figure 1 and Figure 2. The double deletion mutant of chsA and chsC showed septum formation in abnormal locations. The septa were formed closer together in the double deletion mutant compared to the wild type and the single deletion mutants.

challenge
requireschangesYEAST:CHS2Askarlupka, Team JAAM2013-10-11 15:17:18 CDTGO:0005935 cellular bud neck (C)PMID:2968606ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4. Cells that contained a disrupted chs2 gene did not form a septum.

challenge
updatedbyinstructorHELPX:Q9JP55Jluo23, Team JAAM2013-09-29 16:18:09 CDTGO:0070062 extracellular vesicular exosome (C)PMID:20659286ECO:0000314 direct assay evidence used in manual assertion

Figure 8 shows immunogold analysis of CagA, demonstrating localization to bacterial vesicle surface.

challenge
updatedbyinstructorHELPX:P94835Jluo23, Team JAAM2013-09-29 16:09:18 CDTGO:0042000 translocation of peptides or proteins into host (P)PMID:17768234ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1

challenge
requireschangesHELPX:P94835Jluo23, Team JAAM2013-09-29 16:00:53 CDTGO:0072321 chaperone-mediated protein transport (P)PMID:24072713ECO:0000314 direct assay evidence used in manual assertion

Figure 1 shows CagF interaction with CagA using calorimetric analysis; CagF chaperone mediates CagA translocation into gastric epithelial cells.

challenge
unacceptableECOLI:RPOHSchmitz4, Team JAAM2013-09-29 07:07:11 CDTGO:0033554 cellular response to stress (P)PMID:1429436ECO:0000315 mutant phenotype evidence used in manual assertion

figure 8: transforming groESL-containing plasmids into htpR mutant cells showed luminescence induction at a much earlier growth stage than wild-type cells without the plasmid. Luciferase activity of htpR mutant cells was fully recovered by presence of GroESL proteins.

challenge
unacceptableECOLI:RPOHSchmitz4, Team JAAM2013-09-29 07:02:34 CDTGO:0033554 cellular response to stress (P)PMID:1429436ECO:0000315 mutant phenotype evidence used in manual assertion

figure 7 shows that addition of LuxR protein advances luminescence induction of htpR+ cells but has no affect on htpR mutant cells.

challenge
unacceptableECOLI:RPOHSchmitz4, Team JAAM2013-09-29 07:02:34 CDTGO:0033554 cellular response to stress (P)PMID:1429436ECO:0000314 direct assay evidence used in manual assertion

figure 6 shows wild-type cells in stationary phase respond immediately to presence of inducer, whereas wild-type cells of other logarithmic growth phases do not.

challenge
unacceptableECOLI:RPOHSchmitz4, Team JAAM2013-09-29 06:49:01 CDTGO:0033554 cellular response to stress (P)PMID:1429436ECO:0000315 mutant phenotype evidence used in manual assertion

figure 5 presents a typical strong induction of the lux system by added concentrations of a synthetic inducer from Vibrio fischeri. Early induction of the lux system by low concentrations of inducer occurs only when LuxR protein is present and active.

challenge
unacceptableECOLI:RPOHSchmitz4, Team JAAM2013-09-29 06:41:05 CDTGO:0033554 cellular response to stress (P)PMID:1429436ECO:0000315 mutant phenotype evidence used in manual assertion

figure 4: Beta-galactosidase activity increased six times higher in htpR+ cells harboring TRZ plasmid (luxI,luxR, and lacZ). This demonstrates HtpR and its stimulation of LuxR protein synthesis

challenge
updatedbyinstructorALIFS:Q6U738Jluo23, Team JAAM2013-09-15 17:58:45 CDTGO:0035556 intracellular signal transduction (P)PMID:14645281ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 3 demonstrate that one of the Vibrio fischeri GacA global regulator functions is in luminescence. Mutants are defective in luminescence by measuring LU/OD600.

challenge
updatedbyinstructorPHAVU:G1FTR5Jluo23, Team JAAM2013-09-15 17:24:54 CDTGO:0004190 aspartic-type endopeptidase activity (F)PMID:21985276ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2

challenge
acceptablePSEAI:P72137Jluo23, Team JAAM2013-09-15 16:54:53 CDTGO:0030171 voltage-gated proton channel activity (F)PMID:24023388ECO:0000314 direct assay evidence used in manual assertion

I- flux was shown to significantly increase (~200% at pH 6.5 compared to pH 7.5) when proteoliposome external pH was decreased in Fig 2 which indicates Wzx's the proton-dependent transporter activity.

challenge
updatedbyinstructorASPPA:ORDAAskarlupka, Team JAAM2013-09-14 21:46:11 CDTGO:0045122 aflatoxin biosynthetic process (P)PMID:22495451ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2. Mutant phenotype produced no aflatoxin B1 or aflatoxin G1.

challenge

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Pages in category "Team JAAM"

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